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Literature summary for 6.3.4.21 extracted from

  • Galassi, L.; Di Stefano, M.; Brunetti, L.; Orsomando, G.; Amici, A.; Ruggieri, S.; Magni, G.
    Characterization of human nicotinate phosphoribosyltransferase: Kinetic studies, structure prediction and functional analysis by site-directed mutagenesis (2012), Biochimie, 94, 300-309.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ATP at low substrate saturation (below 0,1 mM) Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged protein expressed in Escherichia coli BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D19A activity completely abolished Homo sapiens
D288A about 10% of wild type activity Homo sapiens
G169A about 40% of wild type activity Homo sapiens
G209A about 20% of wild type activity Homo sapiens
G379A activity completely abolished Homo sapiens
H213A about 50% of wild type activity Homo sapiens
N357A about 80% of wild type activity Homo sapiens
R318A activity strictly dependent on the presence of ATP Homo sapiens
S381A about 40% of wild type activity Homo sapiens
T380A about 50% of wild type activity Homo sapiens
Y21A activity strictly dependent on the presence of ATP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA at 1 mM 40% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens
ATP at high substrate saturation Homo sapiens
CoA at 1 mM 70% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens
D-fructose 1,6-bisphosphate at 1 mM 36% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens
glutaryl-CoA at 1 mM 42% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens
glyceraldehyde 3-phosphate at 1 mM 27% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens
phosphoenolpyruvate at 1 mM 33% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens
succinyl-CoA at 1 mM 53% inhibition of activity (concentrations of substrates 1 mM) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0221
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.5, 37°C Homo sapiens
0.0273
-
nicotinate in the presence of phosphate, pH 7.5, 37°C Homo sapiens
0.0382
-
5-phospho-alpha-D-ribose 1-diphosphate in the presence of phosphate, pH 7.5, 37°C Homo sapiens
0.0443
-
nicotinate pH 7.5, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
gel filtration, predicted value of monomer: 59249 Da Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6XQN6
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
Homo sapiens beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
?

Synonyms

Synonyms Comment Organism
nicotinate phosphoribosyltransferase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.01
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.5, 37°C Homo sapiens
0.012
-
nicotinate pH 7.5, 37°C Homo sapiens
0.25
-
nicotinate in the presence of phosphate, pH 7.5, 37°C Homo sapiens
0.36
-
5-phospho-alpha-D-ribose 1-diphosphate in the presence of phosphate, pH 7.5, 37°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.85
-
pH 7.5, 37°C Homo sapiens CoA

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.271
-
nicotinate pH 7.5, 37°C Homo sapiens
0.452
-
5-phospho-alpha-D-ribose 1-diphosphate pH 7.5, 37°C Homo sapiens
9.16
-
nicotinate in the presence of phosphate, pH 7.5, 37°C Homo sapiens
9.42
-
5-phospho-alpha-D-ribose 1-diphosphate in the presence of phosphate, pH 7.5, 37°C Homo sapiens