Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | at low substrate saturation (below 0,1 mM) | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D19A | activity completely abolished | Homo sapiens |
D288A | about 10% of wild type activity | Homo sapiens |
G169A | about 40% of wild type activity | Homo sapiens |
G209A | about 20% of wild type activity | Homo sapiens |
G379A | activity completely abolished | Homo sapiens |
H213A | about 50% of wild type activity | Homo sapiens |
N357A | about 80% of wild type activity | Homo sapiens |
R318A | activity strictly dependent on the presence of ATP | Homo sapiens |
S381A | about 40% of wild type activity | Homo sapiens |
T380A | about 50% of wild type activity | Homo sapiens |
Y21A | activity strictly dependent on the presence of ATP | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | at 1 mM 40% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens | |
ATP | at high substrate saturation | Homo sapiens | |
CoA | at 1 mM 70% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens | |
D-fructose 1,6-bisphosphate | at 1 mM 36% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens | |
glutaryl-CoA | at 1 mM 42% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens | |
glyceraldehyde 3-phosphate | at 1 mM 27% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens | |
phosphoenolpyruvate | at 1 mM 33% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens | |
succinyl-CoA | at 1 mM 53% inhibition of activity (concentrations of substrates 1 mM) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0221 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C | Homo sapiens | |
0.0273 | - |
nicotinate | in the presence of phosphate, pH 7.5, 37°C | Homo sapiens | |
0.0382 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in the presence of phosphate, pH 7.5, 37°C | Homo sapiens | |
0.0443 | - |
nicotinate | pH 7.5, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
gel filtration, predicted value of monomer: 59249 Da | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6XQN6 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Homo sapiens | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinate phosphoribosyltransferase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C | Homo sapiens | |
0.012 | - |
nicotinate | pH 7.5, 37°C | Homo sapiens | |
0.25 | - |
nicotinate | in the presence of phosphate, pH 7.5, 37°C | Homo sapiens | |
0.36 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in the presence of phosphate, pH 7.5, 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.85 | - |
pH 7.5, 37°C | Homo sapiens | CoA |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.271 | - |
nicotinate | pH 7.5, 37°C | Homo sapiens | |
0.452 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C | Homo sapiens | |
9.16 | - |
nicotinate | in the presence of phosphate, pH 7.5, 37°C | Homo sapiens | |
9.42 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in the presence of phosphate, pH 7.5, 37°C | Homo sapiens |