Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.4.21 extracted from

  • Gross, J.W.; Rajavel, M.; Grubmeyer, C.
    Kinetic mechanism of nicotinic acid phosphoribosyltransferase. Implications for energy coupling (1998), Biochemistry, 37, 4189-4199.
    View publication on PubMed
Search for more literature for 6.3.4.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 5-phospho-alpha-D-ribose 1-diphosphate in the coupled reaction, 200fold lower than in the uncoupled reaction Salmonella enterica subsp. enterica serovar Typhimurium
0.33
-
 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.16
-
 ITP good alternative substrate for ATP Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
 ?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O 5-phospho-alpha-D-ribose 1-diphosphate in complex with Mg Salmonella enterica subsp. enterica serovar Typhimurium beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate
-
 ?

Cofactor

Cofactor Comment Organism Structure
ATP reaction accelerated 10-30fold Salmonella enterica subsp. enterica serovar Typhimurium
ITP
-
 Salmonella enterica subsp. enterica serovar Typhimurium