Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
purified QueC, vapor diffusion method, 10 mg/ml protein is mixed with crystallization solution containing 25% PEG 6000, 100 mM NaCl, 10 mM 1,6 hexanediol, and 100 mM MES, pH 5.5, 1 week, X-ray diffraction structure determination and analysis at 2.95 A resolution | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus subtilis | |
Zn2+ | coordination of a zinc ion by the conserved C(x)8CxxCxxC motif in QueC, binding structure, overview | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | O31675 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant QueC from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and another different step of anion exchange chromatography, followed by gel filtration | Bacillus subtilis |
Subunits | Comment | Organism |
---|---|---|
dimer or tetramer | the biologically relevant unit is likely to be a QueC homodimer, crystal structure. Monomer structure, overview. The Rossmann fold of the N-terminal part of QueC, in combination with the C-terminal zinc-binding motif, forms a cavity in QueC of substantial size, which is likely to be the location of substrate binding | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
queC | - |
Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | the products of four genes, queC, queD, queE, and queF, are involved in preQ1 biosynthesis with GTP as the starting material. preQ1 is transformed to Q in tRNA | Bacillus subtilis |