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Literature summary for 6.3.4.20 extracted from

  • McCarty, R.M.; Somogyi, A.; Lin, G.; Jacobsen, N.E.; Bandarian, V.
    The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5-triphosphate in four steps (2009), Biochemistry, 48, 3847-3852.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.20

Cloned(Commentary)

Cloned (Comment) Organism
cloning of His-tagged QueC and expression in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information in vitro preparation of the deazapurine nucleoside, preQ0, by the successive action of the four involved enzymes, i.e. Escherichia coli QueD, Bacillus subtilis QueE and QueC, and Streptomyces rimosus ToyM, cloning and expression in Escherichia coli strain BL21(DE3), overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-carboxy-7-carbaguanine + NH3 + ATP Bacillus subtilis
-
 7-cyano-7-carbaguanine + ADP + phosphate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged QueC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-carboxy-7-carbaguanine + NH3 + ATP
-
 Bacillus subtilis 7-cyano-7-carbaguanine + ADP + phosphate + H2O
-
 ?
7-carboxy-7-carbaguanine + NH3 + ATP dependent conversion of a carboxylic acid to a nitrile Bacillus subtilis 7-cyano-7-carbaguanine + ADP + phosphate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
queC
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus subtilis

General Information

General Information Comment Organism
metabolism the metabolic pathway includes the conversion of the biosynthetic intermediate, 6-carboxy-5,6,7,8-tetrahydropterin, to intermediate, 7-carboxy-7-deazaguanine, CDG, by an unusual transformation catalyzed by QueE, a member of the radical SAM enzyme superfamily. The carboxylate moiety on CDG is converted subsequently to a nitrile to yield preQ0 by QueC in an ATP-dependent reaction, in which ammonia serves as the nitrogen source. CDG may be the central precursor to all deazapurines, biosynthesis of deazapurine containing compounds in nature incorporates H2NTP, CPH4 and CDG as common intermediates Bacillus subtilis