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Literature summary for 6.3.4.2 extracted from

  • Kassel, K.M.; Au, d.a..R.; Higgins, M.J.; Hines, M.; Graves, L.M.
    Regulation of human cytidine triphosphate synthetase 2 by phosphorylation (2010), J. Biol. Chem., 285, 33727-33736.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.2

Protein Variants

Protein Variants Comment Organism
additional information deletion of the C-terminal regulatory domain, residues Ser562-Asp591, of CTPS1 greatly increases the Vmax of the enzyme Homo sapiens
S568A 2fold increase in Km value for UTP. Mutation of S568A significantly increases CTPS2 activity. The S568A mutation has a greater effect on the glutamine than ammonia-dependent activity Homo sapiens
S571A 4fold increase in Km value for UTP Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CTP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information maximally active at physiological concentrations of ATP, GTP, and glutamine, whereas the Km and IC50 values for the substrate UTP and the product CTP, respectively, are close to their physiological concentrations Homo sapiens
0.027
-
 L-Gln wild-type, pH 8.1, 37°C Homo sapiens
0.072
-
 L-Gln mutant S568A, pH 8.1, 37°C Homo sapiens
0.1
-
 L-Gln wild-type, pH 8.1, 37°C Homo sapiens
0.19
-
 UTP wild-type, pH 8.1, 37°C Homo sapiens
0.42
-
 UTP mutant S568A, pH 8.1, 37°C Homo sapiens
0.59
-
 UTP wild-type, pH 8.1, 37°C Homo sapiens
0.8
-
 UTP mutant S571A, pH 8.1, 37°C Homo sapiens
1.9
-
 UTP C-terminal deletion mutant, pH 8.1, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q9NRF8 CTP synthase 2
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Low serum treatment increases CTPS2 phosphorylation. Ser568 and Ser571 are two major phosphorylation sites, and Ser568 is phosphorylated by casein kinase 1 both in vitro and in vivo. Mutation of S568A but not S571A significantly increases CTPS2 activity. The S568A mutation has a greater effect on the glutamine than ammonia-dependent activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UTP + L-Gln
-
 Homo sapiens ADP + phosphate + CTP + L-Glu
-
 ?

Synonyms

Synonyms Comment Organism
CTPS1
-
 Homo sapiens
CTPS2
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.04
-
 wild-type, pH 8.1, 37°C Homo sapiens CTP
0.042
-
 wild-type, pH 8.1, 37°C Homo sapiens CTP
0.045
-
 mutant S571A, pH 8.1, 37°C Homo sapiens CTP
0.057
-
 C-terminal deletion mutant, pH 8.1, 37°C Homo sapiens CTP
0.072
-
 mutant S568A, pH 8.1, 37°C Homo sapiens CTP