BRENDA - Enzyme Database
show all sequences of 6.3.4.2

Structural requirements for the activation of Escherichia coli CTP synthase by the allosteric effector GTP are stringent, but requirements for inhibition are Lax

Lunn, F.A.; Macdonnell, J.E.; Bearne, S.L.; J. Biol. Chem. 283, 2010-2020 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2'-deoxy-GTP
0 mM guanosine, kact: 1.5/sec, KA: 0.21 mM, Ki: 0.36 mM; the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
6-thio-GTP
the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
6-thioguanosine 5'-triphosphate
0 mM guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM
Escherichia coli
GTP
0.1 mM guanosine, kact: 10.3/sec, KA: 0.088 mM, Ki: 0.22 mM; 0.2 mM guanosine, kact: 8.2/sec, KA: 0.078 mM, Ki: 0.12 mM; 0 mM guanosine, kact: 10.6/sec, KA: 0.081 mM, Ki: 0.28 mM; allosteric effector, structural requirements for activation are stringent, but requirements for inhibition are lax. GTP promotes Gln hydrolysis but inhibits Gln-dependent CTP formation at concentrations of over 0.15 mM
Escherichia coli
guanosine 5'-tetraphosphate
0 mM guanosine, kact: 4/sec, KA: 0.19 mM, Ki: 0.5 mM; the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
ITP
0 mM guanosine, kact: 5.2/sec, KA: 2.9 mM, Ki: 4.5 mM; the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
additional information
activation potency in descending order: GTP = 6-thio-GTP, ITP = guanosine 5'-tetraphosphate, O6-methyl-GTP, 2'-deoxy-GTP, no activation with guanosine, GMP, GDP, 2',3'-dideoxy-GTP, acycloguanosine, and acycloguanosine monophosphate, indicating that the 5'-triphosphate, 2'-OH, and 3'-OH are required for full activation, binding structures and kinetics, overview
Escherichia coli
O-methylguanosine 5'-triphosphate
0 mM guanosine, kact: 2.8/sec, KA: 0.13 mM, Ki: 0.29 mM
Escherichia coli
O6-methyl-GTP
the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
Application
Application
Commentary
Organism
drug development
modifying 2-aminopurine or 2-aminopurine riboside may serve as an effective strategy for developing CTPS inhibitors
Escherichia coli
Cloned(Commentary)
Cloned (Commentary)
Organism
cloned in Escherichia coli as a histidine-tagged fusion protein
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
2'-deoxy-GTP
no guanosine, kact: 1.5/sec, KA: 0.21 mM, Ki: 0.36 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
2'-deoxy-guanosine
-
Escherichia coli
2,6-diaminopurine riboside
-
Escherichia coli
2-aminopurine riboside
-
Escherichia coli
3'-deoxy-guanosine
-
Escherichia coli
6-thio-GTP
the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
6-thioguanine
-
Escherichia coli
6-thioguanosine
-
Escherichia coli
6-thioguanosine 5'-triphosphate
no guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM
Escherichia coli
7-deazaguanosine
-
Escherichia coli
8-oxoguanosine
-
Escherichia coli
8-oxoguanosine 5'-triphosphate
-
Escherichia coli
acycloguanosine
-
Escherichia coli
acycloguanosine monophosphate
-
Escherichia coli
adenosine
-
Escherichia coli
dideoxy-GTP
-
Escherichia coli
GDP
-
Escherichia coli
GMP
-
Escherichia coli
GTP
0.1 mM guanosine, kact: 10.3/sec, KA: 0.088 mM, Ki: 0.22 mM; 0.2 mM guanosine, kact: 8.2/sec, KA: 0.078 mM, Ki: 0.12 mM; allosteric effector, structural requirements for activation are stringent, but requirements for inhibition are lax. GTP promotes Gln hydrolysis but inhibits Gln-dependent CTP formation at concentrations of over 0.15 mM; no guanosine, kact: 10.6/sec, KA: 0.081 mM, Ki: 0.28 mM
Escherichia coli
guanosine
-
Escherichia coli
guanosine 5'-tetraphosphate
; no guanosine, kact: 4/sec, KA: 0.19 mM, Ki: 0.5 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
Inosine
-
Escherichia coli
ITP
no guanosine, kact: 5.2/sec, KA: 2.9 mM, Ki: 4.5 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
additional information
GTP analogues inhibite NH3-and Gln-dependent CTP-formation, often in a cooperative manner, to a similar extent as they activate it with IC50 values of 0.2-0.5 mM, the inhibition appears to be due solely to the purine base, binding structures and kinetics, overview. Inhibitor structure-activity study, overview
Escherichia coli
N-methylguanosine
-
Escherichia coli
O-methylguanosine
-
Escherichia coli
O-methylguanosine 5'-triphosphate
no guanosine, kact: 2.8/sec, KA: 0.13 mM, Ki: 0.29 mM
Escherichia coli
O6-methyl-GTP
the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
Xanthosine
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic mechanism, activation and inhibition kinetics
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
; assay with 10 mM MgCl2
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + UTP + L-glutamine
Escherichia coli
-
ADP + phosphate + CTP + L-glutamate
-
-
?
ATP + UTP + NH3
Escherichia coli
-
ADP + phosphate + CTP
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P0A7E5
-
-
Purification (Commentary)
Purification (Commentary)
Organism
the soluble histidine-tagged CTPS is purified using metal ion affinity chromatography and the histidine tag is subsequently removed using thrombin-catalyzed cleavage
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
a structure-activity study using a variety of GTP and guanosine analogues reveals that only a few GTP analogues are capable of activating Gln-dependent CTP formation to varying degrees: GTP > 6-thio-GTP > ITP> guanosine 5'-tetraphosphate > O-methyl-GTP > 2'-deoxy-GTP. No activation is observed with guanosine, GMP, GDP, 2',3'-dideoxy-GTP, acycloguanosine, and acycloguanosine monophosphate indicating that the 5'-triphosphate, 2'-OH, and 3'-OH are required for full activation. The 2-NH2 group is important in binding recognition while substituents at the 6-position are important in activation.
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + UTP + Gln + H2O
-
674909
Escherichia coli
ADP + CTP + Glu + phosphate
-
-
-
?
ATP + UTP + L-glutamine
-
674909
Escherichia coli
ADP + phosphate + CTP + L-glutamate
-
-
-
?
ATP + UTP + NH3
-
674909
Escherichia coli
ADP + CTP + phosphate
-
-
-
?
ATP + UTP + NH3
-
674909
Escherichia coli
ADP + phosphate + CTP
-
-
-
?
deoxyGTP + UTP + NH4+
-
674909
Escherichia coli
deoxyGDP + phosphate + CTP
-
-
-
-
GTP + UTP + NH4+
-
674909
Escherichia coli
GDP + phosphate + CTP
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
CTP synthase
-
Escherichia coli
CTPS
-
Escherichia coli
cytidine 5'-triphosphate synthase
-
Escherichia coli
UTP:ammonia ligase (ADP-forming)
-
Escherichia coli
Temperature Optimum [░C]
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5
-
2'-deoxy-GTP
no guanosine
Escherichia coli
2.8
-
O-methylguanosine 5'-triphosphate
no guanosine
Escherichia coli
4
-
guanosine 5'-tetraphosphate
no guanosine
Escherichia coli
5.2
-
ITP
no guanosine
Escherichia coli
8.2
-
GTP
0.2 mM guanosine
Escherichia coli
8.5
-
6-thioguanosine 5'-triphosphate
no guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM
Escherichia coli
10.3
-
GTP
0.1 mM guanosine
Escherichia coli
10.6
-
GTP
no guanosine
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
dependent on
Escherichia coli
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
kinetic mechanism, activation and inhibition kinetics; nucleotide and nucleoside analogues of GTP and guanosine, respectively, all inhibit NH3- and Gln-dependent CTP formation to a similar extent (IC50 : 0.2ľ0.5 mM). This inhibition appears to be due solely to the purine base and is relatively insensitive to the identity of the purine with the exception of inosine, ITP, and adenosine (IC50 : 4ľ12 mM). 8-Oxoguanosine is the best inhibitor identified (IC50 : 0.08 mM).
Escherichia coli
0.12
-
GTP
0.2 mM guanosine
Escherichia coli
0.22
-
GTP
0.1 mM guanosine
Escherichia coli
0.27
-
6-thioguanosine 5'-triphosphate
-
Escherichia coli
0.28
-
GTP
no guanosine
Escherichia coli
0.29
-
O-methylguanosine 5'-triphosphate
-
Escherichia coli
0.36
-
2'-deoxy-GTP
-
Escherichia coli
0.5
-
guanosine 5'-tetraphosphate
-
Escherichia coli
4.5
-
ITP
-
Escherichia coli
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.08
-
; IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
8-oxoguanosine
0.11
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2,6-diaminopurine riboside
0.11
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
8-oxoguanosine 5'-triphosphate
0.13
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
8-oxoguanosine
0.15
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
8-oxoguanosine 5'-triphosphate
0.15
-
0.30 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
O-methylguanosine
0.16
-
10 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; 2.5 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
O-methylguanosine
0.17
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
N-methylguanosine
0.17
-
0.75 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
O-methylguanosine
0.18
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2,6-diaminopurine riboside
0.22
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2-aminopurine riboside
0.22
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine
0.22
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
Xanthosine
0.23
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
6-thioguanosine
0.23
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.23
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
N-methylguanosine
0.25
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.25
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
O-methylguanosine
0.26
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2-aminopurine riboside
0.26
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.26
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; 2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine
0.28
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.29
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
dideoxy-GTP
0.29
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; 5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GDP
0.29
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.29
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GTP
0.29
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine
0.29
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
Xanthosine
0.3
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GDP
0.3
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; 8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GTP
0.3
-
0.30 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine
0.31
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
acycloguanosine monophosphate
0.31
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GTP
0.32
-
10 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; 2.5 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2'-deoxy-GTP
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2'-deoxy-guanosine
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
acycloguanosine
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
GDP
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
GMP
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine 5'-tetraphosphate
0.34
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
3'-deoxy-guanosine
0.35
-
0.75 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine
0.39
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
6-thioguanine
0.4
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
3'-deoxy-guanosine
0.4
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
6-thioguanosine
0.41
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine monophosphate
0.41
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
dideoxy-GTP
0.42
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2'-deoxy-GTP
0.42
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine 5'-tetraphosphate
0.45
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2'-deoxy-guanosine
0.45
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.47
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.48
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.49
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.61
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
6-thioguanine
2.9
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3.1
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3.3
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3.5
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
Inosine
3.7
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
4.1
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
ITP
5.2
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
Inosine
11
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
adenosine
12
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
adenosine
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2'-deoxy-GTP
0 mM guanosine, kact: 1.5/sec, KA: 0.21 mM, Ki: 0.36 mM; the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
6-thio-GTP
the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
6-thioguanosine 5'-triphosphate
0 mM guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM
Escherichia coli
GTP
0.1 mM guanosine, kact: 10.3/sec, KA: 0.088 mM, Ki: 0.22 mM; 0.2 mM guanosine, kact: 8.2/sec, KA: 0.078 mM, Ki: 0.12 mM; 0 mM guanosine, kact: 10.6/sec, KA: 0.081 mM, Ki: 0.28 mM; allosteric effector, structural requirements for activation are stringent, but requirements for inhibition are lax. GTP promotes Gln hydrolysis but inhibits Gln-dependent CTP formation at concentrations of over 0.15 mM
Escherichia coli
guanosine 5'-tetraphosphate
0 mM guanosine, kact: 4/sec, KA: 0.19 mM, Ki: 0.5 mM; the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
ITP
0 mM guanosine, kact: 5.2/sec, KA: 2.9 mM, Ki: 4.5 mM; the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
additional information
activation potency in descending order: GTP = 6-thio-GTP, ITP = guanosine 5'-tetraphosphate, O6-methyl-GTP, 2'-deoxy-GTP, no activation with guanosine, GMP, GDP, 2',3'-dideoxy-GTP, acycloguanosine, and acycloguanosine monophosphate, indicating that the 5'-triphosphate, 2'-OH, and 3'-OH are required for full activation, binding structures and kinetics, overview
Escherichia coli
O-methylguanosine 5'-triphosphate
0 mM guanosine, kact: 2.8/sec, KA: 0.13 mM, Ki: 0.29 mM
Escherichia coli
O6-methyl-GTP
the GTP analogue is capable of activating Gln-dependent CTP formation
Escherichia coli
Application (protein specific)
Application
Commentary
Organism
drug development
modifying 2-aminopurine or 2-aminopurine riboside may serve as an effective strategy for developing CTPS inhibitors
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
cloned in Escherichia coli as a histidine-tagged fusion protein
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
dependent on
Escherichia coli
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.08
-
; IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
8-oxoguanosine
0.11
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2,6-diaminopurine riboside
0.11
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
8-oxoguanosine 5'-triphosphate
0.13
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
8-oxoguanosine
0.15
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
8-oxoguanosine 5'-triphosphate
0.15
-
0.30 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
O-methylguanosine
0.16
-
10 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; 2.5 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
O-methylguanosine
0.17
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
N-methylguanosine
0.17
-
0.75 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
O-methylguanosine
0.18
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2,6-diaminopurine riboside
0.22
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2-aminopurine riboside
0.22
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine
0.22
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
Xanthosine
0.23
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
6-thioguanosine
0.23
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.23
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
N-methylguanosine
0.25
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.25
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
O-methylguanosine
0.26
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2-aminopurine riboside
0.26
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.26
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; 2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine
0.28
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.29
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
dideoxy-GTP
0.29
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; 5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GDP
0.29
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GMP
0.29
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GTP
0.29
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine
0.29
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
Xanthosine
0.3
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GDP
0.3
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; 8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GTP
0.3
-
0.30 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine
0.31
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
acycloguanosine monophosphate
0.31
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
GTP
0.32
-
10 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; 2.5 mM Gln, IC50 for inhibition of Gln-dependent CTP formation; IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2'-deoxy-GTP
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
2'-deoxy-guanosine
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
acycloguanosine
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
GDP
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
GMP
0.33
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine 5'-tetraphosphate
0.34
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
3'-deoxy-guanosine
0.35
-
0.75 mM Gln, IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
guanosine
0.39
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
6-thioguanine
0.4
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
3'-deoxy-guanosine
0.4
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
6-thioguanosine
0.41
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine monophosphate
0.41
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
dideoxy-GTP
0.42
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2'-deoxy-GTP
0.42
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
guanosine 5'-tetraphosphate
0.45
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
2'-deoxy-guanosine
0.45
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation; IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.47
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.48
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.49
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
acycloguanosine
0.61
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
6-thioguanine
2.9
-
8.16 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3
-
2.72 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3.1
-
5.44 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3.3
-
1.36 mM NH3, IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
3.5
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
Inosine
3.7
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
ITP
4.1
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
ITP
5.2
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
Inosine
11
-
IC50 for inhibition of Gln-dependent CTP formation
Escherichia coli
adenosine
12
-
IC50 for inhibition of NH3-dependent CTP formation
Escherichia coli
adenosine
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2'-deoxy-GTP
no guanosine, kact: 1.5/sec, KA: 0.21 mM, Ki: 0.36 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
2'-deoxy-guanosine
-
Escherichia coli
2,6-diaminopurine riboside
-
Escherichia coli
2-aminopurine riboside
-
Escherichia coli
3'-deoxy-guanosine
-
Escherichia coli
6-thio-GTP
the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
6-thioguanine
-
Escherichia coli
6-thioguanosine
-
Escherichia coli
6-thioguanosine 5'-triphosphate
no guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM
Escherichia coli
7-deazaguanosine
-
Escherichia coli
8-oxoguanosine
-
Escherichia coli
8-oxoguanosine 5'-triphosphate
-
Escherichia coli
acycloguanosine
-
Escherichia coli
acycloguanosine monophosphate
-
Escherichia coli
adenosine
-
Escherichia coli
dideoxy-GTP
-
Escherichia coli
GDP
-
Escherichia coli
GMP
-
Escherichia coli
GTP
0.1 mM guanosine, kact: 10.3/sec, KA: 0.088 mM, Ki: 0.22 mM; 0.2 mM guanosine, kact: 8.2/sec, KA: 0.078 mM, Ki: 0.12 mM; allosteric effector, structural requirements for activation are stringent, but requirements for inhibition are lax. GTP promotes Gln hydrolysis but inhibits Gln-dependent CTP formation at concentrations of over 0.15 mM; no guanosine, kact: 10.6/sec, KA: 0.081 mM, Ki: 0.28 mM
Escherichia coli
guanosine
-
Escherichia coli
guanosine 5'-tetraphosphate
; no guanosine, kact: 4/sec, KA: 0.19 mM, Ki: 0.5 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
Inosine
-
Escherichia coli
ITP
no guanosine, kact: 5.2/sec, KA: 2.9 mM, Ki: 4.5 mM; the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
additional information
GTP analogues inhibite NH3-and Gln-dependent CTP-formation, often in a cooperative manner, to a similar extent as they activate it with IC50 values of 0.2-0.5 mM, the inhibition appears to be due solely to the purine base, binding structures and kinetics, overview. Inhibitor structure-activity study, overview
Escherichia coli
N-methylguanosine
-
Escherichia coli
O-methylguanosine
-
Escherichia coli
O-methylguanosine 5'-triphosphate
no guanosine, kact: 2.8/sec, KA: 0.13 mM, Ki: 0.29 mM
Escherichia coli
O6-methyl-GTP
the GTP analogue is capable of inhibiting Gln-dependent CTP formation at over 0.15 mM
Escherichia coli
Xanthosine
-
Escherichia coli
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
kinetic mechanism, activation and inhibition kinetics; nucleotide and nucleoside analogues of GTP and guanosine, respectively, all inhibit NH3- and Gln-dependent CTP formation to a similar extent (IC50 : 0.2ľ0.5 mM). This inhibition appears to be due solely to the purine base and is relatively insensitive to the identity of the purine with the exception of inosine, ITP, and adenosine (IC50 : 4ľ12 mM). 8-Oxoguanosine is the best inhibitor identified (IC50 : 0.08 mM).
Escherichia coli
0.12
-
GTP
0.2 mM guanosine
Escherichia coli
0.22
-
GTP
0.1 mM guanosine
Escherichia coli
0.27
-
6-thioguanosine 5'-triphosphate
-
Escherichia coli
0.28
-
GTP
no guanosine
Escherichia coli
0.29
-
O-methylguanosine 5'-triphosphate
-
Escherichia coli
0.36
-
2'-deoxy-GTP
-
Escherichia coli
0.5
-
guanosine 5'-tetraphosphate
-
Escherichia coli
4.5
-
ITP
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
kinetic mechanism, activation and inhibition kinetics
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
; assay with 10 mM MgCl2
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + UTP + L-glutamine
Escherichia coli
-
ADP + phosphate + CTP + L-glutamate
-
-
?
ATP + UTP + NH3
Escherichia coli
-
ADP + phosphate + CTP
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
the soluble histidine-tagged CTPS is purified using metal ion affinity chromatography and the histidine tag is subsequently removed using thrombin-catalyzed cleavage
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
a structure-activity study using a variety of GTP and guanosine analogues reveals that only a few GTP analogues are capable of activating Gln-dependent CTP formation to varying degrees: GTP > 6-thio-GTP > ITP> guanosine 5'-tetraphosphate > O-methyl-GTP > 2'-deoxy-GTP. No activation is observed with guanosine, GMP, GDP, 2',3'-dideoxy-GTP, acycloguanosine, and acycloguanosine monophosphate indicating that the 5'-triphosphate, 2'-OH, and 3'-OH are required for full activation. The 2-NH2 group is important in binding recognition while substituents at the 6-position are important in activation.
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + UTP + Gln + H2O
-
674909
Escherichia coli
ADP + CTP + Glu + phosphate
-
-
-
?
ATP + UTP + L-glutamine
-
674909
Escherichia coli
ADP + phosphate + CTP + L-glutamate
-
-
-
?
ATP + UTP + NH3
-
674909
Escherichia coli
ADP + CTP + phosphate
-
-
-
?
ATP + UTP + NH3
-
674909
Escherichia coli
ADP + phosphate + CTP
-
-
-
?
deoxyGTP + UTP + NH4+
-
674909
Escherichia coli
deoxyGDP + phosphate + CTP
-
-
-
-
GTP + UTP + NH4+
-
674909
Escherichia coli
GDP + phosphate + CTP
-
-
-
-
Temperature Optimum [░C] (protein specific)
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.5
-
2'-deoxy-GTP
no guanosine
Escherichia coli
2.8
-
O-methylguanosine 5'-triphosphate
no guanosine
Escherichia coli
4
-
guanosine 5'-tetraphosphate
no guanosine
Escherichia coli
5.2
-
ITP
no guanosine
Escherichia coli
8.2
-
GTP
0.2 mM guanosine
Escherichia coli
8.5
-
6-thioguanosine 5'-triphosphate
no guanosine, kact: 8.5/sec, KA: 0.035 mM, Ki: 0.27 mM
Escherichia coli
10.3
-
GTP
0.1 mM guanosine
Escherichia coli
10.6
-
GTP
no guanosine
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Escherichia coli
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745053
Li
High level of CTP synthase in ...
Mus musculus
Histochem. Cell Biol.
149
61-73
2018
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744847
Huang
Critical roles of CTP synthas ...
Drosophila melanogaster
Exp. Cell Res.
354
122-133
2017
-
-
-
-
5
-
-
-
1
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
744848
Chang
CTP synthase forms the cytoop ...
Drosophila melanogaster, Homo sapiens
Exp. Cell Res.
361
292-299
2017
-
1
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746170
Aughey
The Interplay between Myc and ...
Drosophila melanogaster
PLoS Genet.
12
e1005867
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
745038
Wang
Regulation of CTP synthase fi ...
Drosophila melanogaster
Genetics
201
1511-1523
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727427
Gou
CTP synthase forms cytoophidia ...
Homo sapiens, Mus musculus
Exp. Cell Res.
323
242-253
2014
-
-
2
-
-
-
-
-
4
-
2
-
-
3
-
-
2
-
-
3
-
-
-
2
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
2
-
3
-
-
-
2
-
-
-
-
-
-
-
-
-
2
2
-
-
-
744789
Strochlic
Ack kinase regulates CTP synt ...
Drosophila melanogaster
EMBO Rep.
15
1184-1191
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
745736
Noree
Common regulatory control of ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Mol. Biol. Cell
25
2282-2290
2014
-
-
-
-
7
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
746547
Sandalli
-
Cloning, expression, and char ...
Anoxybacillus gonensis, Anoxybacillus gonensis G2
Turk. J. Biol.
38
111-117
2014
-
-
1
-
-
-
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
1
1
1
2
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
1
1
2
1
1
1
-
-
-
-
-
-
-
-
-
728541
Azzam
Only one isoform of Drosophila ...
Drosophila melanogaster
PLoS Genet.
9
e1003256
2013
-
-
1
-
-
-
-
-
4
-
1
1
-
3
-
-
1
-
-
4
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
1
1
-
-
-
1
-
4
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713658
Lauritsen
Structure of the dimeric form ...
Saccharolobus solfataricus
Acta Crystallogr. Sect. F
67
201-208
2011
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727139
Steeves
Activation and inhibition of C ...
Trypanosoma brucei
Bioorg. Med. Chem. Lett.
21
5188-5190
2011
1
-
1
-
-
-
4
-
-
-
1
1
-
2
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
4
1
-
1
1
-
-
-
4
4
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702613
Roy
Inhibition of CTP synthase fro ...
Escherichia coli
Bioorg. Med. Chem. Lett.
20
141-144
2010
1
1
-
-
-
-
18
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
1
1
-
33
1
1
-
1
-
-
-
33
18
1
1
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
1
1
-
-
-
715549
Kassel
Regulation of human cytidine t ...
Homo sapiens
J. Biol. Chem.
285
33727-33736
2010
-
-
-
-
3
-
1
9
-
-
-
-
-
3
-
1
-
-
-
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Goto
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648966
van Kuilenburg
Identification of a cDNA encod ...
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7
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Verschuur
In vitro inhibition of cytidin ...
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161-169
2000
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Isoforms of human CTP syntheta ...
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648965
Verschuur
Cytidine triphosphate synthase ...
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380
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1999
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648978
Pappas
Characterization of a novel dU ...
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38
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648969
van Kuilenburg
Kinetic properties of CTP synt ...
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648975
Pappas
Nucleotide-dependent tetrameri ...
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J. Biol. Chem.
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648976
Ostrander
Effect of CTP synthetase regul ...
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648994
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Phosphorylation and regulation ...
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648995
Wylie
A single point mutation in CTP ...
Chlamydia trachomatis, Chlamydia trachomatis L2/CPEC
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271
15393-15400
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2
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649000
Wylie
Chlamydia trachomatis CTP synt ...
Chlamydia trachomatis, Escherichia coli
Mol. Microbiol.
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631-642
1996
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7
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648996
Kuilenburg
A fast and novel assay of CTP ...
Bos taurus
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411-416
1994
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648999
Yang
Purification and characterizat ...
Saccharomyces cerevisiae
Biochemistry
33
10785-10793
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2
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649002
Robertson
Characterization of metal ion ...
Escherichia coli
Biochemistry
32
3769-3777
1993
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5
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648997
Lewis
Investigation of the mechanism ...
Escherichia coli
Biochemistry
28
8454-8459
1989
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3
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648998
Kang
Cyclopentenylcytosine triphosp ...
Mus musculus
J. Biol. Chem.
264
713-718
1989
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648980
Weng
Structural role for a conserve ...
Escherichia coli
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169
3023-3028
1987
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649001
Aronow
In situ regulation of mammalia ...
Mus musculus
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262
5106-5112
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649003
Kizaki
Synthesis of N4-substituted CT ...
Mus musculus
Biochem. Biophys. Res. Commun.
145
569-574
1987
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5
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5
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648981
Zalkin
CTP synthetase ...
Escherichia coli, Escherichia coli B / ATCC 11303
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113
282-287
1985
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4
1
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1
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648982
Kizaki
CTP synthetase from Ehrlich as ...
Mus musculus
Biochim. Biophys. Acta
829
34-43
1985
3
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648983
von der Saal
-
Cytidine-5'-triphosphate synth ...
Chlamydia trachomatis, Escherichia coli
J. Am. Chem. Soc.
107
703-704
1985
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2
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648984
Anderson
CTP synthetase from Escherichi ...
Escherichia coli
Biochemistry
22
3285-3292
1983
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1
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648985
Kizaki
Role of GTP in CTP synthetase ...
Mus musculus
Biochem. Biophys. Res. Commun.
108
286-291
1982
1
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648986
Scheit
Substrate specificity of CTP s ...
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Eur. J. Biochem.
126
57-60
1982
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6
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648987
Kizaki
Purification and properties of ...
Mus musculus
Biochim. Biophys. Acta
662
48-54
1981
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648993
Scheit
Substrate specificity of CTP s ...
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229-233
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Weinfeld
CTP synthetase of bovine calf ...
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51
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648989
Long
Cytidine triphosphate syntheta ...
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51
79-83
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Williams
Increased CTP synthetase activ ...
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648991
McPartland
Cytidine 5'-triphosphate synth ...
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1976
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648992
Koshland jr.
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CTP synthetase and related enz ...
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The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
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1974
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