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Literature summary for 6.3.4.2 extracted from

  • Choi, M.G.; Carman, G.M.
    Phosphorylation of human CTP synthetase 1 by protein kinase A: identification of Thr455 as a major site of phosphorylation (2007), J. Biol. Chem., 282, 5367-5377.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.2

Cloned(Commentary)

Cloned (Comment) Organism
human CTP synthetase 1 is expressed and purified from a Saccharomyces cerevisiae ura7delta ura8delta double mutant that lacks CTP synthetase activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
T455A T455A mutation causes a 78% decrease in protein kinase A phosphorylation Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ assay with 10 mM MgCl2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged human CTP synthetase 1 is purified by loading onto a Ni2+-NTA column Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 CTP synthetase 1 activity of the T455A mutant enzyme is 2fold higher than the wild type enzyme. T455A mutation causes a 44% decrease in the amount of human CTP synthetase 1 that is phosphorylated in Saccharomyces cerevisiae cells, accompanied by a 2.5fold increase in the cellular concentration of CTP and a 1.5-fold increase in the choline-dependent synthesis of phosphatidylcholine Homo sapiens
additional information
-
 Thr 455 is identified as a major site of phosphorylation by protein kinase A, phosphorylation at Thr455 results in the inhibition of activity in vitro and in vivo. Data indicate that phosphorylation at Thr455 attenuates the choline-dependent synthesis of phosphatidylcholine when CTP synthetase 1 enzyme is expressed in Saccharomyces cerevisiae Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UTP + Gln + H2O
-
 Homo sapiens ADP + CTP + Glu + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
CTP synthetase 1
-
 Homo sapiens
CTPS1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens