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Literature summary for 6.3.4.2 extracted from

  • Willemös, M.; Molgaard, A.; Johansson, E.; Martinussen, J.
    Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity (2005), FEBS J., 272, 856-864.
    View publication on PubMed
Search for more literature for 6.3.4.2

Activating Compound

Activating Compound Comment Organism Structure
GTP allosteric activator Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
E362Q turnover number for NH4Cl-dependent GTP synthesis reaction is 1.6fold higher than wild-type value Lactococcus lactis
G360A 5fold increase in GTP-dependent activation of uncoupled glutamine hydrolysis compared to wild-type enzyme. Turnover number for NH4Cl-dependent GTP synthesis reaction is 1.2fold higher than wild-type value Lactococcus lactis
G360P mutant enzyme shows no GTP activation of the uncoupled glutaminase reaction, about 4fold lower turnover number for NH4Cl-dependent CTP synthesis reaction than wild-type enzyme Lactococcus lactis
R359M mutant enzyme shows no GTP activation of the uncoupled glutaminase reaction. Turnover number for NH4Cl-dependent GTP synthesis reaction is 1.1fold higher than wild-type value Lactococcus lactis
R359P mutant enzyme shows no GTP activation of the uncoupled glutaminase reaction. Turnover number for NH4Cl-dependent GTP synthesis reaction is 1.2fold lower than wild-type value Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
54
-
 NH4+ 30°C, pH 8.0, wild-type enzyme Lactococcus lactis
56
-
 NH4+ 30°C, pH 8.0, mutant enzyme G360A Lactococcus lactis
57
-
 NH4+ 30°C, pH 8.0, mutant enzyme R359M Lactococcus lactis
78
-
 NH4+ 30°C, pH 8.0, mutant enzyme E362Q Lactococcus lactis
92
-
 NH4+ 30°C, pH 8.0, mutant enzyme G360P Lactococcus lactis
92
-
 NH4+ 30°C, pH 8.0, mutant enzyme R359P Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UTP + NH4+
-
 Lactococcus lactis ADP + phosphate + CTP
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 NH4+ 30°C, pH 8.0, mutant enzyme G360P Lactococcus lactis
5.1
-
 NH4+ 30°C, pH 8.0, mutant enzyme R359P Lactococcus lactis
6.3
-
 NH4+ 30°C, pH 8.0, wild-type enzyme Lactococcus lactis
6.9
-
 NH4+ 30°C, pH 8.0, mutant enzyme R359M Lactococcus lactis
7.5
-
 NH4+ 30°C, pH 8.0, mutant enzyme G360A Lactococcus lactis
10.1
-
 NH4+ 30°C, pH 8.0, mutant enzyme E362Q Lactococcus lactis