Literature summary for 6.3.4.16 extracted from

Popa, E.; Perera, N.; Kibedi-Szabo, C.Z.; Guy-Evans, H.; Evans, D.R.; Purcarea, C.
The smallest active carbamoyl phosphate synthetase was identified in the human gut archaeon Methanobrevibacter smithii (2012), J. Mol. Microbiol. Biotechnol., 22, 287-299.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Methanobrevibacter smithii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.6	1	ATP	at pH 8.0 and 37°C	Methanobrevibacter smithii
1	4.5	HCO3-	at pH 8.0 and 37°C	Methanobrevibacter smithii
15.6	-	NH4+	at pH 8.0 and 37°C	Methanobrevibacter smithii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41403	-	2 * 41403, calculated from amino acid sequence	Methanobrevibacter smithii
44000	-	2 * 44000, SDS-PAGE	Methanobrevibacter smithii
91000	-	gel filtration	Methanobrevibacter smithii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 ATP + NH4+ + HCO3-	Methanobrevibacter smithii	-	2 ADP + phosphate + carbamoyl phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanobrevibacter smithii	A5UK38	DSM 861	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity column chromatography, and gel filtration	Methanobrevibacter smithii

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.00452	-	ATP synthetase reverse reaction rate, at pH 8.0 and 37°C	Methanobrevibacter smithii
0.077	-	carbamoyl phosphate synthetase reaction rate, at pH 8.0 and 37°C	Methanobrevibacter smithii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ATP + NH4+ + HCO3-	-	Methanobrevibacter smithii	2 ADP + phosphate + carbamoyl phosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 44000, SDS-PAGE	Methanobrevibacter smithii
homodimer	2 * 41403, calculated from amino acid sequence	Methanobrevibacter smithii

Synonyms

Synonyms	Comment	Organism
CarB	-	Methanobrevibacter smithii
carbamoyl phosphate synthetase	-	Methanobrevibacter smithii
CPSase	-	Methanobrevibacter smithii
MS-s	-	Methanobrevibacter smithii
Msm_0361	locus name	Methanobrevibacter smithii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.043	-	ATP	at pH 8.0 and 37°C	Methanobrevibacter smithii
0.044	-	HCO3-	at pH 8.0 and 37°C	Methanobrevibacter smithii
0.052	-	NH4+	at pH 8.0 and 37°C	Methanobrevibacter smithii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Methanobrevibacter smithii

pI Value

Organism	Comment	pI Value Maximum	pI Value
Methanobrevibacter smithii	calculated from amino acid sequence	-	4.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.003	-	HCO3-	at pH 8.0 and 37°C	Methanobrevibacter smithii
0.0033	-	NH4+	at pH 8.0 and 37°C	Methanobrevibacter smithii
0.0705	-	ATP	at pH 8.0 and 37°C	Methanobrevibacter smithii

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Release 2023.1 (January 2023)