Activating Compound | Comment | Organism | Structure |
---|---|---|---|
inosine monophosphate | weak activator, the IMP binding site is located at the C-terminus, binding structure analysis from crystal structure with bound IMP, PDB ID 1CE8 | Escherichia coli | |
N-acetyl-L-beta-phenylglutamate | lower activation of CPSI compared to N-acetyl-L-glutamate | Homo sapiens | |
N-acetyl-L-glutamate | i.e. NAG, allosteric activator, binding mode and site structure analysis by MALDI-TOF mass spectrometry, overview | Rattus norvegicus | |
N-acetyl-L-glutamate | i.e. NAG, allosteric activator, the binding mode and site in the crystal structure of human CPSI, PDB ID 2YVQ, is located in the C-terminal domain, docking experiments and structure, overview | Homo sapiens | |
ornithine | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
cloning of N-terminally His6-tagged wild-type and mutant CPSIs in Escherichia coli and expression in Spodopetra frugiperda SF9 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
N1437D | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
N1440D | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
T1391V | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
T1394A | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
W1410K | site-directed mutagenesis, the mutant binds N-acetyl-L-beta-phenylglutamate at the N-acetyl-L-glutamate binding site | Homo sapiens |
W1410K | site-directed mutagenesis, the mutant enzymes shows reduced activation by N-acetyl-L-glutamate compared to the wild-type enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + NH3 + CO2 + H2O | Escherichia coli | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH3 + CO2 + H2O | Rattus norvegicus | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH3 + CO2 + H2O | Homo sapiens | - |
2 ADP + phosphate + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | P31327 | - |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged wild-type and mutant CPSIs from Spodoptera frugiperda SF9 cells by affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + NH3 + CO2 + H2O | - |
Escherichia coli | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH3 + CO2 + H2O | - |
Rattus norvegicus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
2 ATP + NH3 + CO2 + H2O | - |
Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbamoyl phosphate synthetase | - |
Escherichia coli |
carbamoyl phosphate synthetase | - |
Rattus norvegicus |
carbamoyl phosphate synthetase | - |
Homo sapiens |
carbamoyl phosphate synthetase I | - |
Rattus norvegicus |
carbamoyl phosphate synthetase I | - |
Homo sapiens |
CPS | - |
Escherichia coli |
CPSI | - |
Rattus norvegicus |
CPSI | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
7.4 | - |
assay at | Rattus norvegicus |
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Rattus norvegicus | |
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | CPSI is the first urea cycle enzyme | Rattus norvegicus |
metabolism | CPSI is the first urea cycle enzyme | Homo sapiens |
physiological function | CPSI is a key regulator of the urea cycle for ammonia detoxification in animals | Rattus norvegicus |
physiological function | CPSI is a key regulator of the urea cycle for ammonia detoxification in animals, including humans | Homo sapiens |