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Literature summary for 6.3.4.16 extracted from
- Purification, composition, and some properties of rat liver carbamyl phosphate synthetase (ammonia) (1976), Arch. Biochem. Biophys., 175, 270-278.
General Stability
| General Stability | Organism |
|---|---|
| glycerol and DTT stabilize during purification | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial matrix | constitutes 22-26% of the total matrix protein | Rattus norvegicus | 5759 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
2 * 120000, SDS-PAGE | Rattus norvegicus |
| 160000 | 251000 | sedimentation velocity measurement, gel filtration, MW depends on protein concentration and composition of solvent | Rattus norvegicus |
| 222000 | - |
glycerol or sucrose density gradient centrifugation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.87 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 20% v/v glycerol, 0.1 or 2 mM DTT, 48 h, stable | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NH4+ + CO2 + H2O | - |
Rattus norvegicus | ADP + phosphate + carbamoylphosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 120000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
24 h, 20% v/v glycerol, 2 mM DTT, stable | Rattus norvegicus |
| 23 | - |
24 h, 20% v/v glycerol, 2 mM DTT, 13% loss of activity | Rattus norvegicus |
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