Protein Variants | Comment | Organism |
---|---|---|
I11V/P85S/ M157T/K163E/I224T | 17fold increase in the ability to use desthiobiotin as a substrate | Escherichia coli |
M157T | 2-3fold increase in kcat value for desthiobiotin as a substrate | Escherichia coli |
Q41R/M157T/I224T/E215D/T225A | 10fold increase in the ability to use desthiobiotin as a substrate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
desthiobiotin | wild-type, pH 8.3, 30°C | Escherichia coli | |
0.003 | - |
biotin | wild-type, pH 8.3, 30°C | Escherichia coli | |
0.0043 | - |
desthiobiotin | mutant M157T, pH 8.3, 30°C | Escherichia coli | |
0.0067 | - |
desthiobiotin | mutant I11V/P85S/ M157T/K163E/I224T, pH 8.3, 30°C | Escherichia coli | |
0.22 | - |
ATP | wild-type, pH 8.3, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + CGGGSGGGSGLNDIFEAQKIEWH | - |
Escherichia coli | AMP + diphosphate + biotinylated CGGGSGGGSGLNDIFEAQKIEWH | - |
? | |
ATP + desthiobiotin + CGGGSGGGSGLNDIFEAQKIEWH | - |
Escherichia coli | AMP + diphosphate + desthiobiotinylated CGGGSGGGSGLNDIFEAQKIEWH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BirA | - |
Escherichia coli |
BPL | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
desthiobiotin | wild-type, pH 8.3, 30°C | Escherichia coli | |
0.3 | - |
biotin | wild-type, pH 8.3, 30°C | Escherichia coli | |
0.48 | - |
ATP | wild-type, pH 8.3, 30°C | Escherichia coli | |
0.53 | - |
desthiobiotin | mutant M157T, pH 8.3, 30°C | Escherichia coli | |
0.73 | - |
desthiobiotin | mutant I11V/P85S/ M157T/K163E/I224T, pH 8.3, 30°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
desthiobiotin | mutant I11V/P85S/ M157T/K163E/I224T, pH 8.3, 30°C | Escherichia coli | |
120 | - |
desthiobiotin | mutant M157T, pH 8.3, 30°C | Escherichia coli | |
170 | - |
desthiobiotin | wild-type, pH 8.3, 30°C | Escherichia coli | |
1050 | - |
biotin | wild-type, pH 8.3, 30°C | Escherichia coli |