Protein Variants | Comment | Organism |
---|---|---|
E288K | inactive active-site mutant | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | coordinating interactions of active-site metal ions in wild-type and mutant enzymes, Mg2+ is bridged between the alpha- and gamma-phosphoryl group of ATP and coordinated to E288, E276, and a water molecule, detailed overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + CO2 | Escherichia coli | biotin carboxylase catalyzes the ATP-dependent carboxylation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase that catalyzes the first committed step in fatty acid synthesis | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
additional information | Escherichia coli | the reaction mechanism of the acetyl-CoA carboxylase proceeds via two half-reactions: the first half-reaction is catalyzed by biotin carboxylase, the second half-reaction is catalyzed by carboxyltransferase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P24182 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + CO2 | biotin carboxylase catalyzes the ATP-dependent carboxylation of biotin and is one component of the multienzyme complex acetyl-CoA carboxylase that catalyzes the first committed step in fatty acid synthesis | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
ATP + biotin-carboxyl-carrier protein + CO2 | in Gram-negative and Gram-positive bacteria biotin carboxylase, carboxyltransferase, and the biotin carboxyl carrier protein are separate proteins | Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
additional information | the reaction mechanism of the acetyl-CoA carboxylase proceeds via two half-reactions: the first half-reaction is catalyzed by biotin carboxylase, the second half-reaction is catalyzed by carboxyltransferase | Escherichia coli | ? | - |
? | |
additional information | molecular dynamics simulations of wild-type enzyme and active-site mutant E288K, overview | Escherichia coli | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP-dependent | Escherichia coli |