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Literature summary for 6.3.4.14 extracted from

  • Blanchard, C.Z.; Chapman-Smith, A.; Wallace, J.C.; Waldrop, G.L.
    The biotin domain peptide from the biotin carboxyl carrier protein of Escherichia coli acetyl-CoA carboxylase causes a marked increase in the catalytic efficiency of biotin carboxylase and carboxyltransferase relative to free biotin (1999), J. Biol. Chem., 274, 31767-31769.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 biotin-carboxyl-carrier protein C-terminal 87 amino acids of the biotinylated biotin-carboxyl-carrier protein Escherichia coli
84.8
-
 biotin free biotin Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 2 * 50000 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + biotin-carboxyl-carrier protein + HCO3- Escherichia coli first half reaction of the first committed step in the biosynthesis of long-chain fatty acids, in vivo the biotin substrate is attached to biotin-carboxyl-carrier protein, natural substrate ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + HCO3- free biotin, 8000fold lower activity than with the C-terminal 87 amino acids of the biotinylated biotin-carboxyl-carrier protein, biotin carboxylase activity of acetyl-CoA carboxylase Escherichia coli ADP + phosphate + carboxybiotin
-
 ?
ATP + biotin-carboxyl-carrier protein + HCO3- first half reaction of the first committed step in the biosynthesis of long-chain fatty acids, in vivo the biotin substrate is attached to biotin-carboxyl-carrier protein, natural substrate Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
ATP + biotin-carboxyl-carrier protein + HCO3- the biotin carboxylase component of acetyl-CoA carboxylase catalyzes the first half-reaction of the reaction catalyzed by acetyl-CoA carboxylase, the half-reaction involves the phosphorylation of bicarbonate by ATP to form a carboxyphosphate intermediate, followed by transfer of the carboxyl group to biotin to form carboxybiotin, in vivo biotin is attached to biotin-carboxyl-carrier protein, the C-terminal 87 amino acids of the biotinylated biotin-carboxyl-carrier protein form a domain that acts as excellent substrate Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 50000 Escherichia coli

Synonyms

Synonyms Comment Organism
biotin carboxylase (component of acetyl CoA carboxylase)
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.05
-
 biotin Vmax, free biotin Escherichia coli
16.68
-
 biotin-carboxyl-carrier protein Vmax, C-terminal 87 amino acids of the biotinylated biotin-carboxyl-carrier protein Escherichia coli