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Literature summary for 6.3.4.14 extracted from
- Biotin carboxylase component of acetyl-CoA carboxylase from Escherichia coli (1975), Methods Enzymol., 35B, 25-31.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Escherichia coli |  |
| GDP | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
ATP | - |
Escherichia coli | |
| 2.9 | - |
HCO3- | - |
Escherichia coli | |
| 170 | - |
D-(+)-biotin | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Escherichia coli | |
| Mg2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Escherichia coli | |
| Mn2+ | divalent metal ion required, Mg2+, Mn2+ or Co2+ | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration, analytical ultracentrifugation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + HCO3- | ATP in form of MgATP2- | Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
| ATP + biotin-carboxyl-carrier protein + HCO3- | - |
Escherichia coli | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
| ATP + D-(+)-biotin + HCO3- | L-biotin is completely inactive | Escherichia coli | ADP + phosphate + carboxybiotin | - |
? | |
| GTP + biotin-carboxyl-carrier protein + HCO3- | - |
Escherichia coli | GDP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
| GTP + D-biotin + HCO3- | - |
Escherichia coli | GDP + phosphate + carboxybiotin | - |
? | |
| additional information | enzyme catalyzes ATP-hydrolysis in absence of biotin | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
| More | the acetyl-CoA carboxylase system is composed of 3 components: 1. biotin carboxylase, 2. carboxyltransferase, 3. carboxylcarrier protein | Escherichia coli |
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