Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.4.12 extracted from

  • Yamamoto, S.; Morihara, Y.; Wakayama, M.; Tachiki, T.
    Repeated batch production of theanine by coupled fermentation with energy transfer using membrane-enclosed gamma-glutamylmethylamide synthetase and dried yeast cells (2009), Biosci. Biotechnol. Biochem., 73, 2800-2802.
    View publication on PubMed

Application

Application Comment Organism
synthesis the enzyme is enclosed with dried Saccharomyces cerevisiae cells in a dialysis membrane tube to produce theanine from glutamic acid and ethylamine to 100% conversion rate. Six repeats of the reaction are possible in presence of NAD+, overview Methylovorus mays

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain AD494(DE3) Methylovorus mays

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Methylovorus mays

Organism

Organism UniProt Comment Textmining
Methylovorus mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information freeze-dried cells Methylovorus mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamic acid + ethylamine
-
Methylovorus mays theanine i.e. gamma-glutamylethylamide ?

Synonyms

Synonyms Comment Organism
gamma-Glutamylmethylamide synthetase
-
Methylovorus mays
GMAS
-
Methylovorus mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Methylovorus mays