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Literature summary for 6.3.4.10 extracted from
- Distinct amino termini of two human HCS isoforms influence biotin acceptor substrate recognition (2009), J. Biol. Chem., 284, 30862-30870.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the two His-tagged HCS isozymes in Escherichia coli strain Rosetta(DE3) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady state kinetic analyses as a function of biotin, ATP, or a minimal biotin-accepting substrate concentration indicate similar behaviors for both isozymes, pre-steady state kinetic analysis of the second half-reaction with p76 | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
full-length HCS | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + apocarboxylase | Homo sapiens | - |
AMP + diphosphate + holocarboxylase | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
two isozymes that differ at the amino terminus by 57 amino acids | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged HCS isozymes from Escherichia coli strain Rosetta(DE3) | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + biotin + apo-[propionyl-CoA carboxylase] | substrate is p67, the carboxyl-terminal fragment of the propionyl-CoA-carboxylase alpha subunit, the substrate is biotinylated at Lys67 | Homo sapiens | AMP + diphosphate + propionyl-CoA carboxylase | - |
? | |
| ATP + biotin + apocarboxylase | - |
Homo sapiens | AMP + diphosphate + holocarboxylase | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 81000, the two isozymes are monomers both in their apoforms and when bound to the enzymatic intermediate biotinyl 5'-AMP, equilibrium sedimentation centrifugation | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HCS | - |
Homo sapiens |
| Holocarboxylase synthetase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | HCS catalyzes transfer of biotin to biotin-dependent carboxylases, and the enzyme is therefore of fundamental importance for many physiological processes, including fatty acid synthesis, gluconeogenesis, and amino acid catabolism. In addition, the enzyme functions in regulating transcription initiation at several genes that code for proteins involved in biotin metabolism | Homo sapiens |
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Release 2023.1 (January 2023)
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