BRENDA - Enzyme Database show
show all sequences of 6.3.3.6

Stereoselective production of dimethyl-substituted carbapenams via engineered carbapenem biosynthesis enzymes

Hamed, R.; Henry, L.; Claridge, T.; Schofield, C.; ACS Catal. 7, 1279-1285 (2017)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
stereoselctive synthesis of dimethylsubstituted carbapenams using engineered 5-carboxymethylproline synthase and carbapenam-3-carboxylate synthase
Pectobacterium carotovorum
Cloned(Commentary)
Commentary
Organism
-
Pectobacterium carotovorum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pectobacterium carotovorum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2S,6R)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (2S,6S)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2S,3S,5S,6S)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (3R,6R)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2R,3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (3R,6S)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2R,3S,5S,6S)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (3S,6R)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2S,3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
stereoselctive synthesis of dimethylsubstituted carbapenams using engineered 5-carboxymethylproline synthase and carbapenam-3-carboxylate synthase
Pectobacterium carotovorum
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pectobacterium carotovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2S,6R)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (2S,6S)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2S,3S,5S,6S)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (3R,6R)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2R,3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (3R,6S)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2R,3S,5S,6S)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
ATP + (3S,6R)-2,6-dimethyl-t-carboxymethylproline
-
743905
Pectobacterium carotovorum
AMP + diphosphate + (2S,3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate
-
-
-
?
Other publictions for EC 6.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743905
Hamed
-
Stereoselective production of ...
Pectobacterium carotovorum
ACS Catal.
7
1279-1285
2017
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727703
Tahlan
Origins of the beta-lactam rin ...
Pectobacterium carotovorum, Streptomyces cattleya
J. Antibiot.
66
401-410
2013
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Raber
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Pectobacterium carotovorum
Biochemistry
48
4959-40971
2009
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8
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8
8
721605
Arnett
Rate-limiting steps and role o ...
Pectobacterium carotovorum
Biochemistry
46
9337-9345
2007
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1
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2
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1
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1
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1
1
721590
Gerratana
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Pectobacterium carotovorum
Biochemistry
42
7836-7847
2003
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1
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4
4
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2
1
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4
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1
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4
1
1
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4
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9
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4
9
4
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1
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4
1
1
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1
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1
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4
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Pectobacterium carotovorum
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278
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2003
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