BRENDA - Enzyme Database show
show all sequences of 6.3.3.6

Crystal structure of carbapenam synthetase (CarA)

Miller, M.T.; Gerratana, B.; Stapon, A.; Townsend, C.A.; Rosenzweig, A.C.; J. Biol. Chem. 278, 40996-41002 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pectobacterium carotovorum
Crystallization (Commentary)
Crystallization
Organism
sitting drop method, the crystal structures of apo-CarA and CarA complexed with the substrate (2S,5S)-5-carboxymethylproline, ATP analog alpha,beta-methyleneadenosine 5'-triphosphate, and a single Mg2+
Pectobacterium carotovorum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
activity depends on
Pectobacterium carotovorum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55998
-
4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data
Pectobacterium carotovorum
235000
-
gel filtration
Pectobacterium carotovorum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (2S,5S)-5-carboxymethylproline
Pectobacterium carotovorum
the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pectobacterium carotovorum
Q9XB61
-
-
Purification (Commentary)
Commentary
Organism
-
Pectobacterium carotovorum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2S,5S)-5-carboxymethylproline
the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis
722636
Pectobacterium carotovorum
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data
Pectobacterium carotovorum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pectobacterium carotovorum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop method, the crystal structures of apo-CarA and CarA complexed with the substrate (2S,5S)-5-carboxymethylproline, ATP analog alpha,beta-methyleneadenosine 5'-triphosphate, and a single Mg2+
Pectobacterium carotovorum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
activity depends on
Pectobacterium carotovorum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55998
-
4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data
Pectobacterium carotovorum
235000
-
gel filtration
Pectobacterium carotovorum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (2S,5S)-5-carboxymethylproline
Pectobacterium carotovorum
the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pectobacterium carotovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2S,5S)-5-carboxymethylproline
the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis
722636
Pectobacterium carotovorum
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 55998, calculated from sequence, the four monomers form a tetramer with a hole in the center, crystallographic data
Pectobacterium carotovorum
Other publictions for EC 6.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743905
Hamed
-
Stereoselective production of ...
Pectobacterium carotovorum
ACS Catal.
7
1279-1285
2017
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
-
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5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727703
Tahlan
Origins of the beta-lactam rin ...
Pectobacterium carotovorum, Streptomyces cattleya
J. Antibiot.
66
401-410
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
721613
Raber
A conserved tyrosyl-glutamyl c ...
Pectobacterium carotovorum
Biochemistry
48
4959-40971
2009
-
-
-
-
8
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
8
8
721605
Arnett
Rate-limiting steps and role o ...
Pectobacterium carotovorum
Biochemistry
46
9337-9345
2007
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
721590
Gerratana
Inhibition and alternate subst ...
Pectobacterium carotovorum
Biochemistry
42
7836-7847
2003
-
-
1
-
-
-
4
4
-
-
2
1
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
4
1
-
-
-
9
-
-
-
-
1
-
-
-
-
-
4
9
4
-
-
2
1
-
-
-
1
-
-
-
-
4
1
1
-
-
4
1
-
-
-
-
1
1
-
4
4
722636
Miller
Crystal structure of carbapena ...
Pectobacterium carotovorum
J. Biol. Chem.
278
40996-41002
2003
-
-
1
1
-
-
-
-
-
1
2
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
-
-
-
-
-
1
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
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