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show all sequences of 6.3.3.6

Rate-limiting steps and role of active site Lys443 in the mechanism of carbapenam synthetase

Arnett, S.O.; Gerratana, B.; Townsend, C.A.; Biochemistry 46, 9337-9345 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pectobacterium carotovorum
Engineering
Amino acid exchange
Commentary
Organism
K443A
at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity
Pectobacterium carotovorum
K443M
at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity
Pectobacterium carotovorum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pectobacterium carotovorum
-
-
-
Purification (Commentary)
Commentary
Organism
wild-type, K443A and K443M mutant enzymes
Pectobacterium carotovorum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2S,5S)-5-carboxymethylproline
a mechanism is proposed where the rate-limiting step is beta-lactam ring formation coupled to a protein conformational change. The role of K443 throughout the reaction is undercored
721605
Pectobacterium carotovorum
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pectobacterium carotovorum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.36
-
(2S,5S)-5-carboxymethylproline
pH 8.0, 25°, wild-type enzyme
Pectobacterium carotovorum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pectobacterium carotovorum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pectobacterium carotovorum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
K443A
at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity
Pectobacterium carotovorum
K443M
at pH 8.0, 6.33 and 9.33 the mutant enzyme shows no measurable activity
Pectobacterium carotovorum
Purification (Commentary) (protein specific)
Commentary
Organism
wild-type, K443A and K443M mutant enzymes
Pectobacterium carotovorum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (2S,5S)-5-carboxymethylproline
a mechanism is proposed where the rate-limiting step is beta-lactam ring formation coupled to a protein conformational change. The role of K443 throughout the reaction is undercored
721605
Pectobacterium carotovorum
AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Pectobacterium carotovorum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.36
-
(2S,5S)-5-carboxymethylproline
pH 8.0, 25°, wild-type enzyme
Pectobacterium carotovorum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pectobacterium carotovorum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.04
-
(2S,5S)-5-carboxymethylproline
pH 8.0, 25°, wild-type enzyme
Pectobacterium carotovorum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.04
-
(2S,5S)-5-carboxymethylproline
pH 8.0, 25°, wild-type enzyme
Pectobacterium carotovorum
Other publictions for EC 6.3.3.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743905
Hamed
-
Stereoselective production of ...
Pectobacterium carotovorum
ACS Catal.
7
1279-1285
2017
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1
1
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1
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5
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1
1
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-
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5
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-
-
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-
-
-
-
-
-
727703
Tahlan
Origins of the beta-lactam rin ...
Pectobacterium carotovorum, Streptomyces cattleya
J. Antibiot.
66
401-410
2013
-
-
-
-
-
-
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-
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2
-
3
-
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2
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2
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2
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2
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2
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-
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-
-
-
-
-
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-
-
721613
Raber
A conserved tyrosyl-glutamyl c ...
Pectobacterium carotovorum
Biochemistry
48
4959-40971
2009
-
-
-
-
8
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
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-
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-
-
-
8
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
8
8
721605
Arnett
Rate-limiting steps and role o ...
Pectobacterium carotovorum
Biochemistry
46
9337-9345
2007
-
-
1
-
2
-
-
-
-
-
-
-
-
1
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-
1
-
-
-
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1
-
1
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1
1
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-
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1
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2
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1
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-
-
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1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
721590
Gerratana
Inhibition and alternate subst ...
Pectobacterium carotovorum
Biochemistry
42
7836-7847
2003
-
-
1
-
-
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4
4
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-
2
1
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
4
1
-
-
-
9
-
-
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-
1
-
-
-
-
-
4
9
4
-
-
2
1
-
-
-
1
-
-
-
-
4
1
1
-
-
4
1
-
-
-
-
1
1
-
4
4
722636
Miller
Crystal structure of carbapena ...
Pectobacterium carotovorum
J. Biol. Chem.
278
40996-41002
2003
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1
1
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1
2
1
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1
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1
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1
1
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1
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1
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1
2
1
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1
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1
1
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