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Literature summary for 6.3.3.5 extracted from
- . Establishment of an in vitro D-cycloserine-synthesizing system by using O-ureido-L-serine synthase and D-cycloserine synthetase found in the biosynthetic pathway (2013), Antimicrob. Agents Chemother., 57, 2603-2612.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| C-terminally His6-tagged enzyme is overexpression in Escherichia coli | Streptomyces lavendulae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-homocysteine | Streptomyces lavendulae |  |
| 0.037 | - |
ATP | pH 8.0, 30°C, cosubstrate: beta-aminooxy-D-alanine | Streptomyces lavendulae | |
| 0.045 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-2,4-diaminobutyrate | Streptomyces lavendulae | |
| 0.045 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-ornithine | Streptomyces lavendulae | |
| 0.054 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-homoserine | Streptomyces lavendulae | |
| 0.073 | - |
ATP | pH 8.0, 30°C, cosubstrate: O-ureido-D-serine | Streptomyces lavendulae | |
| 10 | - |
O-ureido-D-serine | pH 8.0, 30°C | Streptomyces lavendulae | |
| 19 | - |
D-homocysteine | pH 8.0, 30°C | Streptomyces lavendulae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Streptomyces lavendulae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
gel filtration | Streptomyces lavendulae |
| 34000 | - |
1 * 34000, calculated from sequence | Streptomyces lavendulae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-ureido-D-serine + ATP + H2O | Streptomyces lavendulae | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
| O-ureido-D-serine + ATP + H2O | Streptomyces lavendulae ATCC 11924 | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces lavendulae | D2Z030 | - |
- |
| Streptomyces lavendulae ATCC 11924 | D2Z030 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces lavendulae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-aminooxy-D-alanine + ATP + H2O | - |
Streptomyces lavendulae | D-cycloserine + ? | - |
? | |
| beta-aminooxy-D-alanine + ATP + H2O | - |
Streptomyces lavendulae ATCC 11924 | D-cycloserine + ? | - |
? | |
| D-2,4-diaminobutyrate + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
| D-2,4-diaminobutyrate + ATP + H2O | - |
Streptomyces lavendulae ATCC 11924 | ? | - |
? | |
| D-homocysteine + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
| D-homocysteine + ATP + H2O | - |
Streptomyces lavendulae ATCC 11924 | ? | - |
? | |
| D-homoserine + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
| D-ornithine + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
| O-ureido-D-serine + ATP + H2O | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | Streptomyces lavendulae | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
| O-ureido-D-serine + ATP + H2O | O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O-ureido-L-serine | Streptomyces lavendulae | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
| O-ureido-D-serine + ATP + H2O | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | Streptomyces lavendulae ATCC 11924 | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
| O-ureido-D-serine + ATP + H2O | O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O-ureido-L-serine | Streptomyces lavendulae ATCC 11924 | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, calculated from sequence | Streptomyces lavendulae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DcsG | - |
Streptomyces lavendulae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces lavendulae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 860 | - |
D-homocysteine | pH 8.0, 30°C | Streptomyces lavendulae | |
| 2200 | - |
O-ureido-D-serine | pH 8.0, 30°C | Streptomyces lavendulae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces lavendulae |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
D-ornithine | pH 8.0, 30°C | Streptomyces lavendulae | |
| 2.1 | - |
D-2,4-diaminobutyrate | pH 8.0, 30°C | Streptomyces lavendulae | |
| 6.1 | - |
D-homoserine | pH 8.0, 30°C | Streptomyces lavendulae | |
| 14 | - |
beta-aminooxy-D-alanine | pH 8.0, 30°C | Streptomyces lavendulae | |
| 45 | - |
D-homocysteine | pH 8.0, 30°C | Streptomyces lavendulae | |
| 210 | - |
O-ureido-D-serine | pH 8.0, 30°C | Streptomyces lavendulae | |
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