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Literature summary for 6.3.3.3 extracted from

  • Dey, S.; Lane, J.M.; Lee, R.E.; Rubin, E.J.; Sacchettini, J.C.
    Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase (2010), Biochemistry, 49, 6746-6760.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complexes with the substrate 7,8-diaminopelargonic acid or ADP and the product dethiobiotin, up to 1.85 A resolution. Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
7,8-diaminononanoate pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis
0.029
-
ATP pH 7.5, temperature not specified in the publication Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin enzyme reaction involves three steps: carbamylation of theN7 position of 7,8-diaminononanoate, formation of a carbamate-phosphoric anhydride intermediate, and, closure of the ureido ring to form dethiobiotin and release of the inorganic phosphate Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 7,8-diaminononanoate + CO2
-
Mycobacterium tuberculosis ADP + phosphate + dethiobiotin
-
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