Cloned (Comment) | Organism |
---|---|
wild type and mutant enzymes are expressed in Escherichia coli strain BL21(DE3)/pSJS1244 | Mycoplasma pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
K120A | the mutant shows increased Km and turnover number values for ATP and 5-formyltetrahydrofolate compared to the wild type enzyme | Mycoplasma pneumoniae |
R115A | the mutant shows no activity | Mycoplasma pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
5-formyltetrahydrofolate | wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae | |
0.0071 | - |
5-formyltetrahydrofolate | mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae | |
0.076 | - |
ATP | wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae | |
1.2 | - |
ATP | mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma pneumoniae | P75430 | - |
- |
Purification (Comment) | Organism |
---|---|
Talon metal affinity resin chromatography and Sephadex G-25 gel filtration | Mycoplasma pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | - |
Mycoplasma pneumoniae | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5,10-Methenyltetrahydrofolate synthetase | - |
Mycoplasma pneumoniae |
MTHFS | - |
Mycoplasma pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
5-formyltetrahydrofolate | wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae | |
1 | - |
ATP | wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae | |
1.3 | - |
5-formyltetrahydrofolate | mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae | |
1.7 | - |
ATP | mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C | Mycoplasma pneumoniae |