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Literature summary for 6.3.3.2 extracted from

  • Meier, C.; Carter, L.G.; Winter, G.; Owens, R.J.; Stuart, D.I.; Esnouf, R.M.
    Structure of 5-formyltetrahydrofolate cyclo-ligase from Bacillus anthracis (BA4489) (2007), Acta Crystallogr. Sect. F, F63, 168-172.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli (strain Rosetta pLysS) Bacillus anthracis

Crystallization (Commentary)

Crystallization (Comment) Organism
cocrystallization with folinate, ATP and Mg2+, sitting drop vapour diffusion method using 25% polyethylene glycol 3350, 100 mM bis-Tris pH 5.5 Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81LX0
-
-
Bacillus anthracis BA4489 Q81LX0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity chromatography and gel filtration Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-formyltetrahydrofolate
-
 Bacillus anthracis ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?
ATP + 5-formyltetrahydrofolate
-
 Bacillus anthracis BA4489 ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Bacillus anthracis

Synonyms

Synonyms Comment Organism
5,10-Methenyltetrahydrofolate synthetase
-
 Bacillus anthracis
MTHFS
-
 Bacillus anthracis

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bacillus anthracis