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Literature summary for 6.3.3.2 extracted from

  • Holmes, W.B.; Appling, D.R.
    Cloning and characterization of methenyltetrahydrofolate synthetase from Saccharomyces cerevisiae (2002), J. Biol. Chem., 277, 20205-20213.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene FAU1, orf YER183c, functional expression as His-tagged enzyme in Escherichia coliBL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information disruption mutants do not evoke an altered phenotype Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP- Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
5-formyltetrahydrofolate recombinant enzyme, pH 7.0, 30°C Saccharomyces cerevisiae
0.043
-
ATP recombinant enzyme, pH 7.0, 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-formyltetrahydrofolate Saccharomyces cerevisiae
-
ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity in disruption mutant strains Saccharomyces cerevisiae
7
-
purified recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-formyltetrahydrofolate
-
Saccharomyces cerevisiae ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?
ATP + 5-formyltetrahydrofolate
-
Saccharomyces cerevisiae ADP + phosphate + 5,10-methenyltetrahydrofolate
-
ir

Synonyms

Synonyms Comment Organism
Methenyltetrahydrofolate synthetase
-
Saccharomyces cerevisiae
MTHFS
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
MgATP2- enzyme is inhibited by salt, therefore MgATP2- is used as cofactor instead of MgCl2 and NaATP- Saccharomyces cerevisiae