Cloned (Comment) | Organism |
---|---|
expression of GFP-tagged protein in Tetrahymena thermophila | Tetrahymena thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cilium | main localization, GFP-tagged recombinant protein | Tetrahymena thermophila | 5929 | - |
flagellar basal body | Ttll1 localizes primarily to the basal bodies of oral and somatic cilia | Tetrahymena thermophila | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetrahymena thermophila | I7MCG4 | - |
- |
Tetrahymena thermophila | Q23SI8 | - |
- |
Tetrahymena thermophila SB210 | I7MCG4 | - |
- |
Tetrahymena thermophila SB210 | Q23SI8 | - |
- |
Purification (Comment) | Organism |
---|---|
partial purification of recombinant GFP-tagged Ttll1 and GFP-tagged Ttll9 | Tetrahymena thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-tubulin + L-glutamate | - |
Tetrahymena thermophila | L-glutamyl-alpha-tubulin + ADP + phosphate | - |
? | |
ATP + alpha-tubulin + L-glutamate | - |
Tetrahymena thermophila SB210 | L-glutamyl-alpha-tubulin + ADP + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-tubulin polyglutamylase | - |
Tetrahymena thermophila |
TTHERM_00773080 | - |
Tetrahymena thermophila |
TTL domain protein | - |
Tetrahymena thermophila |
TTLL1 | - |
Tetrahymena thermophila |
TTLL9 | - |
Tetrahymena thermophila |
tubulin glutamylase | - |
Tetrahymena thermophila |
tubulin tyrosine ligase domain protein | - |
Tetrahymena thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tetrahymena thermophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Tetrahymena thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Tetrahymena thermophila |
General Information | Comment | Organism |
---|---|---|
physiological function | Ttll1p glutamylase preferentially modifies alpha-tubulin. It shows a chain-initiating activity. The spatial restriction of modifying enzymes TTLL1 and TTLL9 appears to be a major mechanism that drives differential glutamylation at the subcellular level. Disruption of the isoforms TTLL1 and TTLL9 genes decreases the rates of cell multiplication and phagocytosis. Cells lacking both genes have fewer cortical microtubules and show defects in the maturation of basal bodies. In the TTLL1 and TTLL9 double knockout, a decrease in the levels of glutamylation on alpha-tubulin occurs | Tetrahymena thermophila |
physiological function | Ttll9 glutamylase preferentially modifies alpha-tubulin. It has primarily a chain-elongating activity. The spatial restriction of modifying enzymes TTLL1 and TTLL9 appears to be a major mechanism that drives differential glutamylation at the subcellular level. Disruption of the isoforms TTLL1 and TTLL9 genes decreases the rates of cell multiplication and phagocytosis. Cells lacking both genes have fewer cortical microtubules and show defects in the maturation of basal bodies. In the TTLL1 and TTLL9 double knockout, a decrease in the levels of glutamylation on alpha-tubulin occurs | Tetrahymena thermophila |