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Literature summary for 6.3.2.B19 extracted from

  • Ouchi, T.; Tomita, T.; Horie, A.; Yoshida, A.; Takahashi, K.; Nishida, H.; Lassak, K.; Taka, H.; Mineki, R.; Fujimura, T.; Kosono, S.; Nishiyama, C.; Masui, R.; Kuramitsu, S.; Albers, S.V.; Kuzuyama, T.; Nishiyama, M.
    Lysine and arginine biosyntheses mediated by a common carrier protein in Sulfolobus (2013), Nat. Chem. Biol., 9, 277-283.
    View publication on PubMed
Search for more literature for 6.3.2.B19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfolobus acidocaldarius
expression in Escherichia coli Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
X-ray diffraction with resolution of 1.8 A of the enzyme complexed with LysW, L-glutamate, ADP, Mg2+, Zn2+ and sulfate. X-ray diffraction with resolution of 1.87 A of the enzyme complexed with ADP Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31534
-
 4 * 31534, calculated from sequence Sulfurisphaera tokodaii
31715
-
 x * 31715, calculated from sequence Sulfolobus acidocaldarius
126200
-
 mass spectrometry Sulfurisphaera tokodaii
146000
-
 analytical ultracentrifugation Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfurisphaera tokodaii
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfolobus acidocaldarius arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfolobus acidocaldarius DSM 639 arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate Sulfurisphaera tokodaii 7
-
 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q4J8E7
-
-
Sulfolobus acidocaldarius DSM 639 Q4J8E7
-
-
Sulfurisphaera tokodaii Q970U6
-
-
Sulfurisphaera tokodaii 7 Q970U6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfurisphaera tokodaii ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius DSM 639 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate
-
 Sulfurisphaera tokodaii 7 ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? x * 31715, calculated from sequence Sulfolobus acidocaldarius
tetramer 4 * 31534, calculated from sequence Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
ArgX
-
 Sulfolobus acidocaldarius
Saci_1621 locus name Sulfolobus acidocaldarius
StArgX
-
 Sulfurisphaera tokodaii
STK_15050
-
 Sulfurisphaera tokodaii

General Information

General Information Comment Organism
malfunction no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible Sulfolobus acidocaldarius
physiological function arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW Sulfolobus acidocaldarius