Cloned (Comment) | Organism |
---|---|
produced it in Escherichia coli either unmodified or as a fusion protein with a poly-His tag at the C terminus. Extracts of cells expressing these recombinant proteins display N-acetylaspartylglutamate synthase activity | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
ATP | pH 8.0, 30°C | Mus musculus | |
0.73 | - |
L-glutamate | pH 8.0, 30°C | Mus musculus | |
1.24 | - |
citrate | pH 8.0, 30°C | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + L-glutamate | Mus musculus | the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the immature mammalian brain and in the testis | ADP + phosphate + N-citryl-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q80WS1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
central nervous system | - |
Mus musculus | - |
placenta | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + L-glutamate | the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the immature mammalian brain and in the testis | Mus musculus | ADP + phosphate + N-citryl-L-glutamate | - |
? | |
ATP + citrate + L-glutamate | Vmax/Km for the synthesis of beta-citrylglutamate is 4fold higher than the Vmax/Km value for the synthesis of N-acetylaspartyl-glutamate | Mus musculus | ADP + phosphate + N-citryl-L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-acetylaspartylglutamate synthase | ambiguous | Mus musculus |
NAAG synthetase B | - |
Mus musculus |
NAAGS B | - |
Mus musculus |
RIMKLB | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | from the Vmax, kcat values of 3 and 1.5/s are calculated for the beta-citrylglutamate synthase activity and and N-acetylaspartyl-glutamate synthase activity | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
synthesis of N-citryl-L-glutamate | Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the rates for beta-citrylglutamate synthesis and N-acetylaspartyl-glutamate synthesis are similar between pH 7 and 8, but the beta-citrylglutamate synthetase is severalfold higher than the N-acetylaspartyl-glutamate synthetase activity at more alkaline pH | Mus musculus |
7.5 | 9 | pH 7.5: about 40% of maximal activity, pH 9.0: about 95% of maximal activity, synthesis of N-citryl-L-glutamate | Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the dipeptide N-citryl-L-glutamate, an abundant neuropeptide in the mammalian brain | Mus musculus |