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Literature summary for 6.3.2.9 extracted from

  • Kotnik, M.; Humljan, J.; Contreras-Martel, C.; Oblak, M.; Kristan, K.; Herve, M.; Blanot, D.; Urleb, U.; Gobec, S.; Dessen, A.; Solmajer, T.
    Structural and functional characterization of enantiomeric glutamic acid derivatives as potential transition state analogue inhibitors of MurD ligase (2007), J. Mol. Biol., 370, 107-115.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
vapour-diffusion method and hanging-drop system, high resolution crystal structures of MurD in complexes with two novel inhibitors designed to mimic the transition state of the reaction, N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid or N-(6-butoxy-naphthalene-2-sulfonyl)-Lglutamic acid Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid competitive versus D-Glu, non-competitive versus ATP and UDP-N-acetylmuramoyl-L-Ala Escherichia coli
N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid competitive versus D-Glu, non-competitive versus UDP-N-acetylmuramoyl-L-Ala Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 UDP-N-acetylmuramoyl-L-Ala
-
 Escherichia coli
0.09
-
 D-Glu
-
 Escherichia coli
0.095
-
 ATP
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P14900
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-Ala + Glu highly stereospecific for D-Glu Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu
-
 ?

Synonyms

Synonyms Comment Organism
MurD ligase
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
 N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid versus D-Glu Escherichia coli
0.71
-
 N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid versus UDP-N-acetylmuramoyl-L-Ala Escherichia coli
0.87
-
 N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid versus D-Glu Escherichia coli
1.66
-
 N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid versus ATP Escherichia coli
2.13
-
 N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid versus UDP-N-acetylmuramoyl-L-Ala Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.28
-
-
 Escherichia coli N-(6-butoxy-naphthalene-2-sulfonyl)-D-glutamic acid
0.71
-
-
 Escherichia coli N-(6-butoxy-naphthalene-2-sulfonyl)-L-glutamic acid