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Literature summary for 6.3.2.9 extracted from

  • Bouhss, A.; Dementin, S.; Parquet, C.; Mengin-Lecreulx, D.; Bertrand, J.A.; Le Beller, D.; Dideberg, O.; van Heijenoort, J.; Blanot, D.
    Role of the ortholog and paralog amino acid invariants in the active site of the UDP-MurNAc-L-alanine:D-glutamate ligase (MurD) (1999), Biochemistry, 38, 12240-12247.
    View publication on PubMed
Search for more literature for 6.3.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
D317A reduced enzymatic activity with respect to the wild-type Escherichia coli
D35A reduced enzymatic activity with respect to the wild-type Escherichia coli
E157A reduced enzymatic activity with respect to the wild-type Escherichia coli
E157K reduced enzymatic activity with respect to the wild-type Escherichia coli
H183A reduced enzymatic activity with respect to the wild-type Escherichia coli
H301A reduced enzymatic activity with respect to the wild-type Escherichia coli
K115A reduced enzymatic activity with respect to the wild-type Escherichia coli
K198A reduced enzymatic activity with respect to the wild-type Escherichia coli
K198F reduced enzymatic activity with respect to the wild-type Escherichia coli
N268A reduced enzymatic activity with respect to the wild-type Escherichia coli
N271A reduced enzymatic activity with respect to the wild-type Escherichia coli
R302A reduced enzymatic activity with respect to the wild-type Escherichia coli
R425A reduced enzymatic activity with respect to the wild-type Escherichia coli
Y194F reduced enzymatic activity with respect to the wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, N268A mutant protein Escherichia coli
0.016
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, N271A mutant protein Escherichia coli
0.03
-
 ATP pH 9.4, 37°C, E157A mutant protein Escherichia coli
0.04
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, H301A mutant protein Escherichia coli
0.044
-
 D-glutamate pH 9.4, 37°C, D317A mutant protein Escherichia coli
0.046
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, K115A mutant protein Escherichia coli
0.053
-
 D-glutamate pH 9.4, 37°C, N271A mutant protein Escherichia coli
0.053
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, R425A mutant protein Escherichia coli
0.053
-
 D-glutamate pH 9.4, 37°C, Y194F mutant protein Escherichia coli
0.056
-
 D-glutamate pH 9.4, 37°C, E157K mutant protein Escherichia coli
0.057
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, D317A mutant protein Escherichia coli
0.059
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, H183A mutant protein Escherichia coli
0.065
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, R302A mutant protein Escherichia coli
0.074
-
 D-glutamate pH 9.4, 37°C, R302A mutant protein Escherichia coli
0.084
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, D35A mutant protein Escherichia coli
0.085
-
 ATP pH 9.4, 37°C, E157K mutant protein Escherichia coli
0.095
-
 D-glutamate pH 9.4, 37°C, D35A mutant protein Escherichia coli
0.095
-
 D-glutamate pH 9.4, 37°C, H301A mutant protein Escherichia coli
0.111
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, Y194F mutant protein Escherichia coli
0.114
-
 ATP pH 9.4, 37°C, D35A mutant protein Escherichia coli
0.12
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, K198A mutant protein Escherichia coli
0.128
-
 D-glutamate pH 9.4, 37°C, K115A mutant protein Escherichia coli
0.129
-
 ATP pH 9.4, 37°C, K198F mutant protein Escherichia coli
0.13
-
 ATP pH 9.4, 37°C, H183A mutant protein Escherichia coli
0.135
-
 ATP pH 9.4, 37°C, K198A mutant protein Escherichia coli
0.14
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, K198F mutant protein Escherichia coli
0.14
-
 ATP pH 9.4, 37°C, N268A mutant protein Escherichia coli
0.144
-
 ATP pH 9.4, 37°C, Y194F mutant protein Escherichia coli
0.153
-
 ATP pH 9.4, 37°C, H301A mutant protein Escherichia coli
0.183
-
 ATP pH 9.4, 37°C, R425A mutant protein Escherichia coli
0.185
-
 D-glutamate pH 9.4, 37°C, K198A mutant protein Escherichia coli
0.216
-
 D-glutamate pH 9.4, 37°C, K198F mutant protein Escherichia coli
0.218
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, E157K mutant protein Escherichia coli
0.264
-
 D-glutamate pH 9.4, 37°C, N268A mutant protein Escherichia coli
0.304
-
 UDP-N-acetylmuramoyl L-alanine pH 9.4, 37°C, E157A mutant protein Escherichia coli
0.328
-
 ATP pH 9.4, 37°C, K115A mutant protein Escherichia coli
0.806
-
 D-glutamate pH 9.4, 37°C, H183A mutant protein Escherichia coli
1.58
-
 ATP pH 9.4, 37°C, N271A mutant protein Escherichia coli
2
-
 ATP pH 9.4, 37°C, R302A mutant protein Escherichia coli
2.18
-
 ATP pH 9.4, 37°C, D317A mutant protein Escherichia coli
5.16
-
 D-glutamate pH 9.4, 37°C, R425A mutant protein Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strain DH5alpha
-
Escherichia coli DH5-alpha
-
 strain DH5alpha
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 r
ATP + UDP-N-acetylmuramate-L-alanine + D-glutamate
-
 Escherichia coli DH5-alpha ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 D-glutamate pH 9.4, 37°C, E157K mutant protein Escherichia coli
0.0004
-
 D-glutamate pH 9.4, 37°C, H183A mutant protein Escherichia coli
0.004
-
 D-glutamate pH 9.4, 37°C, K115A mutant protein Escherichia coli
0.012
-
 D-glutamate pH 9.4, 37°C, K198F mutant protein Escherichia coli
0.016
-
 D-glutamate pH 9.4, 37°C, K198A mutant protein Escherichia coli
0.022
-
 D-glutamate pH 9.4, 37°C, D317A mutant protein Escherichia coli
0.093
-
 D-glutamate pH 9.4, 37°C, Y194F mutant protein Escherichia coli
0.3
-
 D-glutamate pH 9.4, 37°C, N271A mutant protein Escherichia coli
0.53
-
 D-glutamate pH 9.4, 37°C, R425A mutant protein Escherichia coli
0.77
-
 D-glutamate pH 9.4, 37°C, H301A mutant protein Escherichia coli
3 6 D-glutamate pH 9.4, 37°C, R302A mutant protein Escherichia coli
3.13
-
 D-glutamate pH 9.4, 37°C, D35A mutant protein Escherichia coli
3.13
-
 D-glutamate pH 9.4, 37°C, R302A mutant protein Escherichia coli
3.3
-
 D-glutamate pH 9.4, 37°C, N268A mutant protein Escherichia coli
4.27
-
 D-glutamate pH 9.4, 37°C, D35A mutant protein Escherichia coli
5.12
-
 D-glutamate pH 9.4, 37°C, N268A mutant protein Escherichia coli
6.08
-
 D-glutamate pH 9.4, 37°C, H301A mutant protein Escherichia coli
6.08
-
 D-glutamate pH 9.4, 37°C, R425A mutant protein Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.4
-
-
 Escherichia coli