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Literature summary for 6.3.2.8 extracted from

  • Hesse, L.; Bostock, J.; Dementin, S.; Blanot, D.; Mengin-Lecreulx, D.; Chopra, I.
    Functional and biochemical analysis of Chlamydia trachomatis MurC, an enzyme displaying UDP-N-acetylmuramate:amino acid ligase activity (2003), J. Bacteriol., 185, 6507-6512.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.8

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate 20% stimulation at concentrations above 25mM Chlamydia trachomatis

Cloned(Commentary)

Cloned (Comment) Organism
functionally expressed in Escherichia coli H1119, temperature-sensitive, using the pET30a vector Chlamydia trachomatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.124
-
 L-alanine 37°C, pH 8.6 Chlamydia trachomatis
0.242
-
 L-serine 37°C, pH 8.6 Chlamydia trachomatis
1.17
-
 glycine 37°C, pH 8.6 Chlamydia trachomatis

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 dependent with maximal enzymatic activity at 20mM MgCl2 Chlamydia trachomatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57760
-
 calculated for the His- and S-tagged enzyme Chlamydia trachomatis
57760
-
 mass spectrometry of the recombinant enzyme Chlamydia trachomatis
65000
-
 gel filtration of the recombinant enzyme Chlamydia trachomatis

Organism

Organism UniProt Comment Textmining
Chlamydia trachomatis
-
-
-
Chlamydia trachomatis L2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
method that includes chromatography using a Ni-nitrilotriacetic acid Superflow agarose-filled column and gel filtration Chlamydia trachomatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.074
-
 37°C, pH 8.6 Chlamydia trachomatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramate + glycine
-
 Chlamydia trachomatis ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
 r
ATP + UDP-N-acetylmuramate + glycine
-
 Chlamydia trachomatis L2 ADP + phosphate + UDP-N-acetylmuramoyl-glycine
-
 r
ATP + UDP-N-acetylmuramate + L-alanine
-
 Chlamydia trachomatis ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 r
ATP + UDP-N-acetylmuramate + L-alanine
-
 Chlamydia trachomatis L2 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine
-
 r
ATP + UDP-N-acetylmuramate + L-serine
-
 Chlamydia trachomatis ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 r
ATP + UDP-N-acetylmuramate + L-serine
-
 Chlamydia trachomatis L2 ADP + phosphate + UDP-N-acetylmuramoyl-L-serine
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Chlamydia trachomatis