General Stability | Organism |
---|---|
presence of 2-mercaptoethanol and glycerol is essential for stability | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,3,3-Trifluoro-DL-Ala | - |
Escherichia coli | |
3,3-Difluoro-L-Ala | - |
Escherichia coli | |
3-chloro-L-Ala | - |
Escherichia coli | |
3-Cyano-L-Ala | - |
Escherichia coli | |
3-Fluoro-L-Ala | - |
Escherichia coli | |
L-2-aminobutyrate | - |
Escherichia coli | |
L-Allylglycine | weak | Escherichia coli | |
L-Cys | weak | Escherichia coli | |
L-propargylglycine | - |
Escherichia coli | |
L-Vinylglycine | - |
Escherichia coli | |
potassium phosphate | no effect up to 20 mM, significant decrease of activity at higher concentrations | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-Ala | - |
Escherichia coli | |
0.1 | - |
UDP-N-acetylmuramate | alpha anomer of UDP-N-acetylmuramate | Escherichia coli | |
0.43 | - |
UDP-N-acetylglucosyl-enolpyruvate | - |
Escherichia coli | |
0.45 | - |
ATP | - |
Escherichia coli | |
1 | - |
dihydro-UDP-N-acetylmuramate | - |
Escherichia coli | |
2 | - |
UDP-N-acetyl-beta-D-muramate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration: 10-20 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
overproduced in strains harbouring the recombinant plasmids bearing the mur gene under the control of the lac promoter | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.94 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 20 mM potassium phosphate, pH 7.2, 1 mM EDTA, 2.5 mM 2-mercaptoethanol, 15% glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + dihydro-UDP-N-acetylmuramate + L-Ala | - |
Escherichia coli | ADP + phosphate + dihydro-UDP-N-acetylmuramoyl-L-Ala | - |
? | |
ATP + UDP-N-acetyl-beta-D-muramate + L-alanine | - |
Escherichia coli | ADP + phosphate + UDP-N-acetyl-beta-D-muramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetylglucosaminyl-enoylpyruvate + L-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylglucosaminyl-enoylpyruvoyl-L-Ala | - |
? | |
ATP + UDP-N-acetylglucosyl-enolpyruvate + L-alanine | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylglucosyl-enolpyruvoyl-L-alanine | - |
? | |
ATP + UDP-N-acetylmuramate + DL-Ser | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser | - |
? | |
ATP + UDP-N-acetylmuramate + Gly | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-Gly | - |
? | |
ATP + UDP-N-acetylmuramate + L-Ala | r | Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? | |
ATP + UDP-N-acetylmuramate + L-Ala | alpha- and beta-anomer of UDP-N-acetylmuramate | Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? | |
additional information | enzyme catalyzes an exchange reaction leading to the formation of 4% of the UDP-N-acetylmuramoyl-L-[14C]Ala with UDP-N-acetylmuramoyl-L-Ala, L-[14C]Ala, ADP and phosphate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
- |
Escherichia coli |