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Literature summary for 6.3.2.8 extracted from

  • Liger, S.; Masson, A.; Blanot, D.; van Heijenoort, J.; Parquet, C.
    Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramate:L-alanine ligase from Escherichia coli (1995), Eur. J. Biochem., 230, 80-87.
    View publication on PubMed
Search for more literature for 6.3.2.8

General Stability

General Stability Organism
presence of 2-mercaptoethanol and glycerol is essential for stability Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
3,3,3-Trifluoro-DL-Ala
-
 Escherichia coli
3,3-Difluoro-L-Ala
-
 Escherichia coli
3-chloro-L-Ala
-
 Escherichia coli
3-Cyano-L-Ala
-
 Escherichia coli
3-Fluoro-L-Ala
-
 Escherichia coli
L-2-aminobutyrate
-
 Escherichia coli
L-Allylglycine weak Escherichia coli
L-Cys weak Escherichia coli
L-propargylglycine
-
 Escherichia coli
L-Vinylglycine
-
 Escherichia coli
potassium phosphate no effect up to 20 mM, significant decrease of activity at higher concentrations Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 L-Ala
-
 Escherichia coli
0.1
-
 UDP-N-acetylmuramate alpha anomer of UDP-N-acetylmuramate Escherichia coli
0.43
-
 UDP-N-acetylglucosyl-enolpyruvate
-
 Escherichia coli
0.45
-
 ATP
-
 Escherichia coli
1
-
 dihydro-UDP-N-acetylmuramate
-
 Escherichia coli
2
-
 UDP-N-acetyl-beta-D-muramate
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 10-20 mM Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 overproduced in strains harbouring the recombinant plasmids bearing the mur gene under the control of the lac promoter
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.94
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 20 mM potassium phosphate, pH 7.2, 1 mM EDTA, 2.5 mM 2-mercaptoethanol, 15% glycerol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dihydro-UDP-N-acetylmuramate + L-Ala
-
 Escherichia coli ADP + phosphate + dihydro-UDP-N-acetylmuramoyl-L-Ala
-
 ?
ATP + UDP-N-acetyl-beta-D-muramate + L-alanine
-
 Escherichia coli ADP + phosphate + UDP-N-acetyl-beta-D-muramoyl-L-alanine
-
 ?
ATP + UDP-N-acetylglucosaminyl-enoylpyruvate + L-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylglucosaminyl-enoylpyruvoyl-L-Ala
-
 ?
ATP + UDP-N-acetylglucosyl-enolpyruvate + L-alanine
-
 Escherichia coli ADP + phosphate + UDP-N-acetylglucosyl-enolpyruvoyl-L-alanine
-
 ?
ATP + UDP-N-acetylmuramate + DL-Ser
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser
-
 ?
ATP + UDP-N-acetylmuramate + Gly
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala r Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
ATP + UDP-N-acetylmuramate + L-Ala alpha- and beta-anomer of UDP-N-acetylmuramate Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
 ?
additional information enzyme catalyzes an exchange reaction leading to the formation of 4% of the UDP-N-acetylmuramoyl-L-[14C]Ala with UDP-N-acetylmuramoyl-L-Ala, L-[14C]Ala, ADP and phosphate Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Escherichia coli