KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
L-Ala | - |
Escherichia coli | |
0.039 | - |
UDP-N-acetylmuramate | - |
Escherichia coli | |
0.14 | - |
ATP | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
maltose binding-protein-UDP-N-acetylmuramyl:L-alanine ligase fusion protein is overproduced in Escherichia coli/pMal::murC | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | ligase-catalyzed peptide formation proceeds through an activated acyl-phosphate linkage during the reaction process. ATP assists in this process of the peptide bond formation by donating its gamma-phosphoryl group to activate the carboxyl group of UDP-N-acetylmuramate | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate + L-Ala | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? |