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Literature summary for 6.3.2.7 extracted from

  • Anwar, R.A.; Vlaovic, M.
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysine synthetase from Bacillus sphaericus: activation by potassium phosphate (1986), Biochem. Cell Biol., 64, 297-303.
    View publication on PubMed
Search for more literature for 6.3.2.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 Lys in presence of 30 mM K2HPO4 Lysinibacillus sphaericus
0.42
-
 Lys in presence of 2 mM K2HPO4 Lysinibacillus sphaericus
0.44
-
 Lys in presence of 60 mM KCl Lysinibacillus sphaericus
0.49
-
 Lys in presence of 60 mM KCl Lysinibacillus sphaericus
0.52
-
 ATP in presence of 2 mM K2HPO4 Lysinibacillus sphaericus
0.53
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu in presence of 30 mM K2HPO4 Lysinibacillus sphaericus
0.55
-
 ATP in presence of 30 mM K2HPO4 Lysinibacillus sphaericus
0.57
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu in presence of 2 mM K2HPO4 Lysinibacillus sphaericus
0.59
-
 UDP-N-acetylmuramoyl-L-Ala-D-Glu in presence of 60 mM KCl Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- activation by different anions in decreasing order: HPO42-, Cl-, SO42- , potassium salts give higher activity than the corresponding sodium salts Lysinibacillus sphaericus
HPO42- activation by different anions in decreasing order: HPO42-, Cl-, SO42- , potassium salts give higher activity than the corresponding sodium salts Lysinibacillus sphaericus
Mg2+ required Lysinibacillus sphaericus
Mg2+ optimal concentration: 10 mM Lysinibacillus sphaericus
phosphate the presence of phosphate changes the enzyme conformation to a catalytically more active form Lysinibacillus sphaericus
SO42- activation by different anions in decreasing order: HPO42-, Cl-, SO42- , potassium salts give higher activity than the corresponding sodium salts Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys Lysinibacillus sphaericus one step in the biosynthesis of bacterial cell walls ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
 enzyme is synthesized during vegetative growth
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus sphaericus

Source Tissue

Source Tissue Comment Organism Textmining
vegetative
-
 Lysinibacillus sphaericus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.245
-
-
 Lysinibacillus sphaericus

Storage Stability

Storage Stability Organism
-20°C, stable for over 3 months when stored in the lyophilized form Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys
-
 Lysinibacillus sphaericus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys
-
 ?
ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu + L-Lys one step in the biosynthesis of bacterial cell walls Lysinibacillus sphaericus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Lysinibacillus sphaericus

pH Range

pH Minimum pH Maximum Comment Organism
8 8.5 activity decreases sharply below pH 8.0, optimal activity at pH 8.5 Lysinibacillus sphaericus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 rapid loss of activity at or below, even at 0°C Lysinibacillus sphaericus
8
-
 stable at Lysinibacillus sphaericus