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Literature summary for 6.3.2.51 extracted from

  • Kupke, T.
    Active-site residues and amino acid specificity of the bacterial 4'-phosphopantothenoylcysteine synthetase CoaB (2004), Eur. J. Biochem., 271, 163-172.
    View publication on PubMed
Search for more literature for 6.3.2.51

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
A276V the mutation affects enzyme activity Escherichia coli
N210D the mutation affects enzyme activity Escherichia coli
R206Q the mutation affects enzyme activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-cysteine Escherichia coli
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrapQ column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Escherichia coli AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Escherichia coli CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
additional information the wild-type enzyme is not able to couple L-serine, L-alanine or DL-homocysteine to (R)-4'-phosphopantothenate Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
4'-phosphopantothenoylcysteine synthetase
-
 Escherichia coli
CoaB
-
 Escherichia coli
Dfp
-
 Escherichia coli
PPC-synthetase
-
 Escherichia coli