BRENDA - Enzyme Database show
show all sequences of 6.3.2.50

Biosynthesis of the mycotoxin tenuazonic acid by a fungal NRPS-PKS hybrid enzyme

Yun, C.S.; Motoyama, T.; Osada, H.; Nat. Commun. 6, 8758 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in the protease-deficient Saccharomyces cerevisiae strain BJ5464
Magnaporthe oryzae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
173000
-
-
Magnaporthe oryzae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-isoleucine + acetoacetyl-CoA
Magnaporthe oryzae
-
AMP + diphosphate + tenuazonic acid + CoA
-
-
?
ATP + L-isoleucine + acetoacetyl-CoA
Magnaporthe oryzae kita 1
-
AMP + diphosphate + tenuazonic acid + CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Magnaporthe oryzae
-
-
-
Magnaporthe oryzae kita 1
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-isoleucine + acetoacetyl-CoA
-
741052
Magnaporthe oryzae
AMP + diphosphate + tenuazonic acid + CoA
-
-
-
?
ATP + L-isoleucine + acetoacetyl-CoA
-
741052
Magnaporthe oryzae kita 1
AMP + diphosphate + tenuazonic acid + CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 173000, SDS-PAGE
Magnaporthe oryzae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in the protease-deficient Saccharomyces cerevisiae strain BJ5464
Magnaporthe oryzae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
173000
-
-
Magnaporthe oryzae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-isoleucine + acetoacetyl-CoA
Magnaporthe oryzae
-
AMP + diphosphate + tenuazonic acid + CoA
-
-
?
ATP + L-isoleucine + acetoacetyl-CoA
Magnaporthe oryzae kita 1
-
AMP + diphosphate + tenuazonic acid + CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-isoleucine + acetoacetyl-CoA
-
741052
Magnaporthe oryzae
AMP + diphosphate + tenuazonic acid + CoA
-
-
-
?
ATP + L-isoleucine + acetoacetyl-CoA
-
741052
Magnaporthe oryzae kita 1
AMP + diphosphate + tenuazonic acid + CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 173000, SDS-PAGE
Magnaporthe oryzae
Other publictions for EC 6.3.2.50
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741052
Yun
Biosynthesis of the mycotoxin ...
Magnaporthe oryzae, Magnaporthe oryzae kita 1
Nat. Commun.
6
8758
2015
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-