1.5
L-alanine
pH 7.1, 37°C, mutant enzyme Y75F
Bacillus subtilis
1.7
L-alanine
pH 7.7, 37°C, mutant enzyme Y75F
Bacillus subtilis
2
L-alanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
2.6
L-alanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
4.6
L-alanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
4.9
L-alanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
16
L-phenylalanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
18
L-phenylalanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
38
L-phenylalanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
44
L-phenylalanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
45
L-phenylalanine
pH 7.7, 37°C, mutant enzyme
Bacillus subtilis
92
L-phenylalanine
pH 7.1, 37°C, mutant enzyme
Bacillus subtilis
ATP + L-alanine + (1R,2S,6R)-anticapsin
Bacillus subtilis
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
ATP + L-alanine + (1R,2S,6R)-anticapsin
Bacillus subtilis 168
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog
730896
Bacillus subtilis
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
?
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog
730896
Bacillus subtilis 168
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
?
ATP + L-alanine + (1R,2S,6R)-anticapsin
730896
Bacillus subtilis
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
ATP + L-alanine + (1R,2S,6R)-anticapsin
730896
Bacillus subtilis 168
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
ATP + L-alanine + L-phenylalanine
the substrate pair L-alanine and L-phenylalanine shows the highest activity among the combinations of less bulky amino acids (glycine, L-alanine, and L-serine) and uncharged bulky amino acids (L-leucine, L-isolucine, L-methionine, L-phenylalanine, L-tyrosine, L-tryptophane, and L-glutamine). Some D-amino acids (D-alanine, D-serine, D-phenylalanine, and D-glutamine) are tested but show no activity
730896
Bacillus subtilis
ADP + phosphate + L-alanyl-L-phenylalanine
?
ATP + L-alanine + L-phenylalanine
the substrate pair L-alanine and L-phenylalanine shows the highest activity among the combinations of less bulky amino acids (glycine, L-alanine, and L-serine) and uncharged bulky amino acids (L-leucine, L-isolucine, L-methionine, L-phenylalanine, L-tyrosine, L-tryptophane, and L-glutamine). Some D-amino acids (D-alanine, D-serine, D-phenylalanine, and D-glutamine) are tested but show no activity
730896
Bacillus subtilis 168
ADP + phosphate + L-alanyl-L-phenylalanine
?
7.2
L-phenylalanine
pH 7.1, 37°C, mutant enzyme
Bacillus subtilis
7.2
L-alanine
pH 7.1, 37°C, mutant enzyme Y75F
Bacillus subtilis
7.7
L-alanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
7.7
L-phenylalanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
7.8
L-alanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
7.8
L-phenylalanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
9.7
L-phenylalanine
pH 7.7, 37°C, mutant enzyme
Bacillus subtilis
9.7
L-alanine
pH 7.7, 37°C, mutant enzyme Y75F
Bacillus subtilis
9.7
L-alanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
9.7
L-phenylalanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
10.8
L-alanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
10.8
L-phenylalanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
1.5
L-alanine
pH 7.1, 37°C, mutant enzyme Y75F
Bacillus subtilis
1.7
L-alanine
pH 7.7, 37°C, mutant enzyme Y75F
Bacillus subtilis
2
L-alanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
2.6
L-alanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
4.6
L-alanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
4.9
L-alanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
16
L-phenylalanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
18
L-phenylalanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
38
L-phenylalanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
44
L-phenylalanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
45
L-phenylalanine
pH 7.7, 37°C, mutant enzyme
Bacillus subtilis
92
L-phenylalanine
pH 7.1, 37°C, mutant enzyme
Bacillus subtilis
ATP + L-alanine + (1R,2S,6R)-anticapsin
Bacillus subtilis
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
ATP + L-alanine + (1R,2S,6R)-anticapsin
Bacillus subtilis 168
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog
730896
Bacillus subtilis
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
?
(2S)-3-[[(1R)-1-aminoethyl(hydroxy)]phosphoryl]-2-benzylpropionic acid + ATP
the phosphinate L-alanyl-L-phenylalanine analog binds the active site of the enzyme, and accepts a phosphate group from ATP to form the phosphorylated phosphinate L-alanyl-L-phenylalanine analog
730896
Bacillus subtilis 168
(2S)-3-[(3)-[(1R)-1-aminoethyl(phosphonooxy)]phosphoryl]-2-benzylpropanoic acid + ADP
?
ATP + L-alanine + (1R,2S,6R)-anticapsin
730896
Bacillus subtilis
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
ATP + L-alanine + (1R,2S,6R)-anticapsin
730896
Bacillus subtilis 168
ADP + phosphate + bacilysin
bacilysin i.e. L-alanyl-3-[(1R,2S,6R)-5-oxo-7-oxabicyclo[4.1.0]hept-2-yl]-L-alanine
?
ATP + L-alanine + L-phenylalanine
the substrate pair L-alanine and L-phenylalanine shows the highest activity among the combinations of less bulky amino acids (glycine, L-alanine, and L-serine) and uncharged bulky amino acids (L-leucine, L-isolucine, L-methionine, L-phenylalanine, L-tyrosine, L-tryptophane, and L-glutamine). Some D-amino acids (D-alanine, D-serine, D-phenylalanine, and D-glutamine) are tested but show no activity
730896
Bacillus subtilis
ADP + phosphate + L-alanyl-L-phenylalanine
?
ATP + L-alanine + L-phenylalanine
the substrate pair L-alanine and L-phenylalanine shows the highest activity among the combinations of less bulky amino acids (glycine, L-alanine, and L-serine) and uncharged bulky amino acids (L-leucine, L-isolucine, L-methionine, L-phenylalanine, L-tyrosine, L-tryptophane, and L-glutamine). Some D-amino acids (D-alanine, D-serine, D-phenylalanine, and D-glutamine) are tested but show no activity
730896
Bacillus subtilis 168
ADP + phosphate + L-alanyl-L-phenylalanine
?
7.2
L-phenylalanine
pH 7.1, 37°C, mutant enzyme
Bacillus subtilis
7.2
L-alanine
pH 7.1, 37°C, mutant enzyme Y75F
Bacillus subtilis
7.7
L-alanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
7.7
L-phenylalanine
pH 7.1, 37°C, wild-type enzyme
Bacillus subtilis
7.8
L-alanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
7.8
L-phenylalanine
pH 7.1, 37°C, mutant enzyme S184F
Bacillus subtilis
9.7
L-phenylalanine
pH 7.7, 37°C, mutant enzyme
Bacillus subtilis
9.7
L-alanine
pH 7.7, 37°C, mutant enzyme Y75F
Bacillus subtilis
9.7
L-alanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
9.7
L-phenylalanine
pH 7.7, 37°C, wild-type enzyme
Bacillus subtilis
10.8
L-alanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
10.8
L-phenylalanine
pH 7.7, 37°C, mutant enzyme S184A
Bacillus subtilis
733347
Tsuda
Single mutation alters the sub ...
Bacillus subtilis
Biochemistry
53
2650-2660
2014
-
1
-
1
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726743
Arai
L-amino acid ligase from Pseud ...
Pseudomonas syringae, Pseudomonas syringae NBRC 14081
Appl. Environ. Microbiol.
79
5023-5029
2013
-
1
1
-
-
-
-
-
-
1
-
4
-
5
-
-
-
-
-
-
-
-
100
-
3
1
1
-
-
1
1
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
100
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
729244
Parker
Action and timing of BacC and ...
Bacillus subtilis, Bacillus subtilis 168
Biochemistry
52
889-901
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
8
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726581
Suzuki
The structure of L-amino-acid ...
Bacillus licheniformis, Bacillus licheniformis NBRC 12200
Acta Crystallogr. Sect. D
68
1535-1540
2012
-
-
1
1
-
-
-
-
-
1
-
2
-
6
-
-
1
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726615
Tsuda
Crystallization and preliminar ...
Bacillus subtilis, Bacillus subtilis 168
Acta Crystallogr. Sect. F
68
203-206
2012
-
-
-
1
1
-
-
-
-
-
2
-
-
9
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730896
Shomura
Structural and enzymatic chara ...
Bacillus subtilis, Bacillus subtilis 168
Protein Sci.
21
707-716
2012
-
1
1
1
2
-
1
12
-
-
-
2
-
6
-
-
1
-
-
-
-
-
6
1
1
1
-
-
12
1
-
-
-
1
-
-
-
1
1
-
1
2
-
-
1
1
12
-
-
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
12
1
-
-
-
-
-
-
-
-
-
702768
Kino
A novel L-amino acid ligase fr ...
Bacillus subtilis, Bacillus subtilis NBRC3134
Biosci. Biotechnol. Biochem.
74
129-134
2010
-
-
1
-
-
-
-
-
-
1
-
-
-
6
-
-
-
-
-
-
-
-
2
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
702771
Senoo
Identification of novel L-amin ...
Actinobacillus pleuropneumoniae, Streptococcus mutans, Streptococcus pneumoniae, Treponema denticola, Photorhabdus laumondii subsp. laumondii
Biosci. Biotechnol. Biochem.
74
415-418
2010
-
-
5
-
-
-
-
-
-
-
-
-
-
5
-
-
5
-
-
-
-
-
115
-
10
5
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
115
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
690733
Kino
Dipeptide synthesis by L-amino ...
Ralstonia solanacearum, Ralstonia solanacearum JCM 10489
Biochem. Biophys. Res. Commun.
371
536-540
2008
-
-
1
-
-
-
-
1
-
1
1
2
-
6
-
-
1
-
-
-
-
-
4
1
2
1
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
1
2
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
693286
Kino
A novel L-amino acid ligase fr ...
Bacillus licheniformis, Bacillus licheniformis NBRC12200
J. Biosci. Bioeng.
106
313-315
2008
-
-
1
-
-
-
-
-
-
1
-
4
-
8
-
-
1
-
-
-
-
-
17
-
2
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
17
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671498
Tabata
Fermentative production of L-a ...
Bacillus subtilis
Appl. Environ. Microbiol.
73
6378-6385
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
662037
Tabata
ywf in Bacillus subtilis codes ...
Bacillus subtilis, Bacillus subtilis 168
J. Bacteriol.
187
5195-5202
2005
-
-
1
-
-
-
2
3
-
2
1
-
-
8
-
-
1
-
-
-
-
-
4
1
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
3
-
2
1
-
-
-
-
1
-
-
-
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
733219
Steinborn
bac genes for recombinant baci ...
Bacillus pumilus, no activity in Bacillus licheniformis, no activity in Bacillus megaterium, no activity in Bacillus coagulans, no activity in Bacillus pumilus, no activity in Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus subtilis 168, Bacillus pumilus ATCC 7065, Bacillus amyloliquefaciens ATCC 15841, no activity in Bacillus amyloliquefaciens GSB272, Bacillus subtilis A1/3, no activity in Bacillus licheniformis ATCC 9789, no activity in Bacillus megaterium PV361, no activity in Bacillus pumilus ATCC 12140
Arch. Microbiol.
183
71-79
2005
-
-
4
-
1
-
-
-
-
4
-
8
-
21
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
4
-
1
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
7
7
-
-
-