Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.49 extracted from

  • Kino, K.; Nakazawa, Y.; Yagasaki, M.
    Dipeptide synthesis by L-amino acid ligase from Ralstonia solanacearum (2008), Biochem. Biophys. Res. Commun., 371, 536-540.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lal, expression of His-tagged enzyme in Escherichia coli Ralstonia solanacearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Ralstonia solanacearum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Ralstonia solanacearum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49500
-
x * 49500, recombinant His-tagged enzyme, SDS-PAGE Ralstonia solanacearum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + an L-amino acid + an L-amino acid Ralstonia solanacearum
-
ADP + phosphate + L-aminoacyl-L-amino acid
-
?
ATP + an L-amino acid + an L-amino acid Ralstonia solanacearum JCM 10489
-
ADP + phosphate + L-aminoacyl-L-amino acid
-
?

Organism

Organism UniProt Comment Textmining
Ralstonia solanacearum B2DEW2 gene lal
-
Ralstonia solanacearum JCM 10489 B2DEW2 gene lal
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Ralstonia solanacearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + an L-amino acid + an L-amino acid
-
Ralstonia solanacearum ADP + phosphate + L-aminoacyl-L-amino acid
-
?
ATP + an L-amino acid + an L-amino acid substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview Ralstonia solanacearum ADP + phosphate + L-aminoacyl-L-amino acid
-
?
ATP + an L-amino acid + an L-amino acid
-
Ralstonia solanacearum JCM 10489 ADP + phosphate + L-aminoacyl-L-amino acid
-
?
ATP + an L-amino acid + an L-amino acid substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview Ralstonia solanacearum JCM 10489 ADP + phosphate + L-aminoacyl-L-amino acid
-
?

Subunits

Subunits Comment Organism
? x * 49500, recombinant His-tagged enzyme, SDS-PAGE Ralstonia solanacearum

Synonyms

Synonyms Comment Organism
LAL
-
Ralstonia solanacearum
RSp1486a
-
Ralstonia solanacearum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Ralstonia solanacearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Ralstonia solanacearum

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Ralstonia solanacearum

Cofactor

Cofactor Comment Organism Structure
ATP
-
Ralstonia solanacearum