Cloned (Comment) | Organism |
---|---|
gene lal, expression of His-tagged enzyme in Escherichia coli | Ralstonia solanacearum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Ralstonia solanacearum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Ralstonia solanacearum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49500 | - |
x * 49500, recombinant His-tagged enzyme, SDS-PAGE | Ralstonia solanacearum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + an L-amino acid + an L-amino acid | Ralstonia solanacearum | - |
ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | Ralstonia solanacearum JCM 10489 | - |
ADP + phosphate + L-aminoacyl-L-amino acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia solanacearum | B2DEW2 | gene lal | - |
Ralstonia solanacearum JCM 10489 | B2DEW2 | gene lal | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Ralstonia solanacearum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + an L-amino acid + an L-amino acid | - |
Ralstonia solanacearum | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview | Ralstonia solanacearum | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | - |
Ralstonia solanacearum JCM 10489 | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? | |
ATP + an L-amino acid + an L-amino acid | substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview | Ralstonia solanacearum JCM 10489 | ADP + phosphate + L-aminoacyl-L-amino acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49500, recombinant His-tagged enzyme, SDS-PAGE | Ralstonia solanacearum |
Synonyms | Comment | Organism |
---|---|---|
LAL | - |
Ralstonia solanacearum |
RSp1486a | - |
Ralstonia solanacearum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ralstonia solanacearum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Ralstonia solanacearum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Ralstonia solanacearum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Ralstonia solanacearum |