Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CdSO4 | 2 mM, complete inhibition | Bacillus subtilis | |
ZnSO4 | 2 mM, complete inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-glutamine | pH 9.0, 37°C | Bacillus subtilis | |
0.42 | - |
ATP | pH 9.0, 37°C | Bacillus subtilis | |
105 | - |
L-alanine | pH 9.0, 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ is essential for activity | Bacillus subtilis | |
Mn2+ | Mg2+ or Mn2+ is essential for activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P39641 | - |
- |
Bacillus subtilis 168 | P39641 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + L-glutamine | GTP does not substitute for ATP | Bacillus subtilis | ADP + phosphate + L-alanyl-L-glutamine | - |
? | |
ATP + L-alanine + L-glutamine | GTP does not substitute for ATP | Bacillus subtilis 168 | ADP + phosphate + L-alanyl-L-glutamine | - |
? | |
additional information | while the enzyme has extremely broad substrate specificity, some preferences are observed; the enzyme does not accept highly charged amino acids such as Lys, Arg, Glu, and Asp. The enzyme does not react with secondary amines such as Pro. The N-terminal residue of the dipeptide formed seems to be limited to Ala, Gly, Ser, Thr, and Met whereas the C-terminal residue seems to allow for a wider array of amino acids; and Ala and Ser seems to be most preferred for the N-terminal residue while Met and Phe seems to be preferred for the C-terminus. The enzyme does not react with D-Ala, D-serine, or D-phenylalanine | Bacillus subtilis | ? | - |
? | |
additional information | while the enzyme has extremely broad substrate specificity, some preferences are observed; the enzyme does not accept highly charged amino acids such as Lys, Arg, Glu, and Asp. The enzyme does not react with secondary amines such as Pro. The N-terminal residue of the dipeptide formed seems to be limited to Ala, Gly, Ser, Thr, and Met whereas the C-terminal residue seems to allow for a wider array of amino acids; and Ala and Ser seems to be most preferred for the N-terminal residue while Met and Phe seems to be preferred for the C-terminus. The enzyme does not react with D-Ala, D-serine, or D-phenylalanine | Bacillus subtilis 168 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
YwfE | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | 25°C: about 70% of maximal activity, 45°C: about 55% of maximal activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 10.5 | pH 8.5: about 70% of maximal activity, pH 10.5: about 35% of maximal activity | Bacillus subtilis |