BRENDA - Enzyme Database
show all sequences of 6.3.2.49

bac genes for recombinant bacilysin and anticapsin production in Bacillus host strains

Steinborn, G.; Hajirezaei, M.R.; Hofemeister, J.; Arch. Microbiol. 183, 71-79 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
Bacillus amyloliquefaciens
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322; gene bacD, genetic structure of gene cluster bacABCDE, complementation study with bacD genes from several other Bacillus sources, overview
Bacillus subtilis
gene bacD, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
Bacillus pumilus
Engineering
Amino acid exchange
Commentary
Organism
additional information
several bacilysin-deficient strains, e.g. strain ng79, construction of mutant strains GSB315, GSB319, GSB320, GSB321, and GSB322
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bacillus amyloliquefaciens
Mg2+
-
Bacillus pumilus
Mg2+
;
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-alanine + L-anticapsin
Bacillus pumilus
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus subtilis
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus amyloliquefaciens
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus subtilis 168
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus pumilus ATCC 7065
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus amyloliquefaciens ATCC 15841
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus subtilis A1/3
-
ADP + phosphate + bacilysin
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus amyloliquefaciens
Q8KWS8
with plasmid pSB657, gene bacD
-
Bacillus amyloliquefaciens ATCC 15841
Q8KWS8
with plasmid pSB657, gene bacD
-
Bacillus pumilus
-
gene bacD
-
Bacillus pumilus ATCC 7065
-
gene bacD
-
Bacillus subtilis
P39641
with plasmid pSB657, gene bacD or ywfE, gene cluster ywfBCDEF or bacABCDE
-
Bacillus subtilis
Q8KWT3
with plasmid pSB657, gene bacD
-
Bacillus subtilis 168
P39641
with plasmid pSB657, gene bacD or ywfE, gene cluster ywfBCDEF or bacABCDE
-
Bacillus subtilis A1/3
Q8KWT3
with plasmid pSB657, gene bacD
-
no activity in Bacillus amyloliquefaciens
-
-
-
no activity in Bacillus amyloliquefaciens GSB272
-
-
-
no activity in Bacillus coagulans
-
-
-
no activity in Bacillus licheniformis
-
-
-
no activity in Bacillus licheniformis ATCC 9789
-
-
-
no activity in Bacillus megaterium
-
-
-
no activity in Bacillus megaterium PV361
-
-
-
no activity in Bacillus pumilus
-
-
-
no activity in Bacillus pumilus ATCC 12140
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-alanine + L-anticapsin
-
733219
Bacillus pumilus
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus subtilis
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus amyloliquefaciens
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus subtilis 168
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus pumilus ATCC 7065
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus amyloliquefaciens ATCC 15841
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus subtilis A1/3
ADP + phosphate + bacilysin
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus amyloliquefaciens
ATP
-
Bacillus pumilus
ATP
;
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
Bacillus amyloliquefaciens
gene bacD, DNA and amino acid sequence determination, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
Bacillus subtilis
gene bacD, genetic structure of gene cluster bacABCDE, complementation study with bacD genes from several other Bacillus sources, overview
Bacillus subtilis
gene bacD, recombinant functional expression in enzyme-deficient Bacillus subtilis strains GSB315 or GSB322
Bacillus pumilus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Bacillus amyloliquefaciens
ATP
-
Bacillus pumilus
ATP
-
Bacillus subtilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
several bacilysin-deficient strains, e.g. strain ng79, construction of mutant strains GSB315, GSB319, GSB320, GSB321, and GSB322
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Bacillus amyloliquefaciens
Mg2+
-
Bacillus pumilus
Mg2+
-
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + L-alanine + L-anticapsin
Bacillus pumilus
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus subtilis
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus amyloliquefaciens
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus subtilis 168
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus pumilus ATCC 7065
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus amyloliquefaciens ATCC 15841
-
ADP + phosphate + bacilysin
-
-
?
ATP + L-alanine + L-anticapsin
Bacillus subtilis A1/3
-
ADP + phosphate + bacilysin
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + L-alanine + L-anticapsin
-
733219
Bacillus pumilus
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus subtilis
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus amyloliquefaciens
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus subtilis 168
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus pumilus ATCC 7065
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus amyloliquefaciens ATCC 15841
ADP + phosphate + bacilysin
-
-
-
?
ATP + L-alanine + L-anticapsin
-
733219
Bacillus subtilis A1/3
ADP + phosphate + bacilysin
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant
Bacillus amyloliquefaciens
malfunction
mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant
Bacillus subtilis
additional information
physiological prevention of suicidal antibiotic effects, e.g. for enzyme-deficient strain GSB322 with plasmid pSB674(bacABCD), overview
Bacillus subtilis
physiological function
the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae
Bacillus amyloliquefaciens
physiological function
the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae
Bacillus pumilus
physiological function
the enzyme shows antibiotic activity against various indicator strains as Candida albicans CAP1-312, Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae; the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae
Bacillus subtilis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant
Bacillus amyloliquefaciens
malfunction
mutation of the bacD gene or transformation of the bacABC genes into a Bacillus subtilis D (ywfAbacABCDE) deletion mutant leads to the accumulation of anticapsin, which is 4fold higher after transformation of the bacABC genes into a bacD mutant
Bacillus subtilis
additional information
physiological prevention of suicidal antibiotic effects, e.g. for enzyme-deficient strain GSB322 with plasmid pSB674(bacABCD), overview
Bacillus subtilis
physiological function
the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae
Bacillus amyloliquefaciens
physiological function
the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae
Bacillus pumilus
physiological function
the enzyme shows antibiotic activity against various indicator strains as Candida albicans CAP1-312, Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Sthapylococcus aureus, Streptomyces typhimurium, or Saccharomces cerevisiae
Bacillus subtilis
physiological function
the enzyme shows antibiotic activity against various indicator strains as Escherica coli B, Escherichia coli K12, Proteus mirabilis, Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, or Saccharomyces cerevisiae
Bacillus subtilis
Other publictions for EC 6.3.2.49
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733347
Tsuda
Single mutation alters the sub ...
Bacillus subtilis
Biochemistry
53
2650-2660
2014
-
1
-
1
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726743
Arai
L-amino acid ligase from Pseud ...
Pseudomonas syringae, Pseudomonas syringae NBRC 14081
Appl. Environ. Microbiol.
79
5023-5029
2013
-
1
1
-
-
-
-
-
-
1
-
4
-
4
-
-
-
-
-
-
-
-
100
-
1
1
-
-
1
1
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
100
-
1
1
-
-
1
1
-
-
-
-
-
-
-
-
729244
Parker
Action and timing of BacC and ...
Bacillus subtilis 168, Bacillus subtilis
Biochemistry
52
889-901
2013
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
1
-
-
-
-
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8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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2
-
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1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726581
Suzuki
The structure of L-amino-acid ...
Bacillus licheniformis, Bacillus licheniformis NBRC 12200
Acta Crystallogr. Sect. D
68
1535-1540
2012
-
-
1
1
-
-
-
-
-
1
-
2
-
4
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726615
Tsuda
Crystallization and preliminar ...
Bacillus subtilis 168, Bacillus subtilis
Acta Crystallogr. Sect. F
68
203-206
2012
-
-
-
1
1
-
-
-
-
-
2
-
-
7
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730896
Shomura
Structural and enzymatic chara ...
Bacillus subtilis 168, Bacillus subtilis
Protein Sci.
21
707-716
2012
-
1
1
1
2
-
1
12
-
-
-
2
-
6
-
-
1
-
-
-
-
-
6
1
1
-
-
10
1
-
-
-
1
-
-
-
1
1
-
1
2
-
-
1
1
12
-
-
-
2
-
-
-
1
-
-
-
-
6
1
1
-
-
10
1
-
-
-
-
-
-
-
-
-
702768
Kino
A novel L-amino acid ligase fr ...
Bacillus subtilis, Bacillus subtilis NBRC3134
Biosci. Biotechnol. Biochem.
74
129-134
2010
-
-
1
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
1
1
-
-
1
1
-
-
-
1
1
-
-
-
702771
Senoo
Identification of novel L-amin ...
Actinobacillus pleuropneumoniae, Photorhabdus laumondii subsp. laumondii, Streptococcus mutans, Streptococcus pneumoniae, Treponema denticola
Biosci. Biotechnol. Biochem.
74
415-418
2010
-
-
5
-
-
-
-
-
-
-
-
-
-
5
-
-
5
-
-
-
-
-
115
-
5
-
-
-
5
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
115
-
5
-
-
-
5
-
-
-
-
-
-
-
-
-
690733
Kino
Dipeptide synthesis by L-amino ...
Ralstonia solanacearum, Ralstonia solanacearum JCM 10489
Biochem. Biophys. Res. Commun.
371
536-540
2008
-
-
1
-
-
-
-
1
-
1
1
2
-
4
-
-
1
-
-
-
-
-
4
1
1
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
1
1
2
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
1
-
-
-
-
-
-
-
-
693286
Kino
A novel L-amino acid ligase fr ...
Bacillus licheniformis, Bacillus licheniformis NBRC12200
J. Biosci. Bioeng.
106
313-315
2008
-
-
1
-
-
-
-
-
-
1
-
4
-
4
-
-
1
-
-
-
-
-
17
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
17
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671498
Tabata
Fermentative production of L-a ...
Bacillus subtilis
Appl. Environ. Microbiol.
73
6378-6385
2007
-
1
-
-
-
-
-
-
-
-
-
-
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2
-
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1
-
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-
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-
-
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-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
662037
Tabata
ywf in Bacillus subtilis codes ...
Bacillus subtilis, Bacillus subtilis 168
J. Bacteriol.
187
5195-5202
2005
-
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1
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2
3
-
2
1
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7
-
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1
-
-
-
-
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4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
3
-
2
1
-
-
-
-
1
-
-
-
-
4
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
733219
Steinborn
bac genes for recombinant baci ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens ATCC 15841, Bacillus pumilus, Bacillus pumilus ATCC 7065, Bacillus subtilis, Bacillus subtilis 168, Bacillus subtilis A1/3, no activity in Bacillus amyloliquefaciens, no activity in Bacillus amyloliquefaciens GSB272, no activity in Bacillus coagulans, no activity in Bacillus licheniformis, no activity in Bacillus licheniformis ATCC 9789, no activity in Bacillus megaterium, no activity in Bacillus megaterium PV361, no activity in Bacillus pumilus, no activity in Bacillus pumilus ATCC 12140
Arch. Microbiol.
183
71-79
2005
-
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3
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1
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-
-
3
-
8
-
21
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
3
-
-
-
-
-
4
4
-
1
-
-
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
6
7
-
-
-