BRENDA - Enzyme Database show
show all sequences of 6.3.2.47

Matthews, M.L.; Bollinger, J.M.Jr.; Kelleher, N.L.; Walsh, C.T.: The nonribosomal peptide synthetase enzyme DdaD tethers N(beta)-fumaramoyl-l-2,3-diaminopropionate for Fe(II)/alpha-ketoglutarate-dependent epoxidation by DdaC during dapdiamide antibiotic biosynthesis

Hollenhorst, M.A.; Bumpus, S.B.; J. Am. Chem. Soc. 132, 15773-15781 (2010)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine
pH 8.0, 22C
Pantoea agglomerans
0.072
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine
pH 8.0, 22C
Pantoea agglomerans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pantoea agglomerans
E2JA31
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine + L-valine
i.e. N-beta-epoxysuccinamoyl-2,3-diaminopropionate. DdaF can catalyze the ligation of both Nbeta-trans-epoxysuccinamoyl-diaminopropionate diastereomers to Val to produce the Nbeta-trans-epoxysuccinamoyl-diaminopropionate-L-valine dipeptide antibiotics. DdaF uses (R,R)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate as a saturable substrate, whereas saturation is not achieved with (S,S)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate at concentrations up to 590 microM
729882
Pantoea agglomerans
ADP + phosphate + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanyl-L-valine
product is a natural antibiotic
-
-
?
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-valine
i.e. Nbeta-fumaramoyl-diaminopropionate
729882
Pantoea agglomerans
ADP + phosphate + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanyl-L-valine
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine
pH 8.0, 22C
Pantoea agglomerans
3
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine
pH 8.0, 22C
Pantoea agglomerans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.053
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine
pH 8.0, 22C
Pantoea agglomerans
0.072
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine
pH 8.0, 22C
Pantoea agglomerans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine + L-valine
i.e. N-beta-epoxysuccinamoyl-2,3-diaminopropionate. DdaF can catalyze the ligation of both Nbeta-trans-epoxysuccinamoyl-diaminopropionate diastereomers to Val to produce the Nbeta-trans-epoxysuccinamoyl-diaminopropionate-L-valine dipeptide antibiotics. DdaF uses (R,R)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate as a saturable substrate, whereas saturation is not achieved with (S,S)-Nbeta-trans-epoxysuccinamoyl-diaminopropionate at concentrations up to 590 microM
729882
Pantoea agglomerans
ADP + phosphate + 3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanyl-L-valine
product is a natural antibiotic
-
-
?
ATP + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine + L-valine
i.e. Nbeta-fumaramoyl-diaminopropionate
729882
Pantoea agglomerans
ADP + phosphate + 3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanyl-L-valine
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.3
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine
pH 8.0, 22C
Pantoea agglomerans
3
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine
pH 8.0, 22C
Pantoea agglomerans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.83
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine
pH 8.0, 22C
Pantoea agglomerans
56.7
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine
pH 8.0, 22C
Pantoea agglomerans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.83
-
3-[[(2E)-4-amino-4-oxobut-2-enoyl]amino]-L-alanine
pH 8.0, 22C
Pantoea agglomerans
56.7
-
3-((((2R,3R)-3-carboxyoxiran-2-yl)carbonyl)amino)-L-alanine
pH 8.0, 22C
Pantoea agglomerans
Other publictions for EC 6.3.2.47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729882
Hollenhorst
Matthews, M.L.; Bollinger, J.M ...
Pantoea agglomerans
J. Am. Chem. Soc.
132
15773-15781
2010
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729202
Hollenhorst
The ATP-dependent amide ligase ...
Pantoea agglomerans
Biochemistry
48
10467-10472
2009
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