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show all sequences of 6.3.2.46

The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics

Hollenhorst, M.A.; Clardy, J.; Walsh, C.T.; Biochemistry 48, 10467-10472 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pantoea agglomerans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
fumarate
pH 8.0, 22C
Pantoea agglomerans
20
-
succinate
pH 8.0, 22C
Pantoea agglomerans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + fumarate + (2S)-2,3-diaminopropionate
Pantoea agglomerans
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pantoea agglomerans
E2JA32
-
-
Purification (Commentary)
Commentary
Organism
-
Pantoea agglomerans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + fumaramate + (2S)-2,3-diaminopropionate
activity with fumaramate is about 15% of the activity compared to fumarate
729202
Pantoea agglomerans
?
-
-
-
?
ATP + fumarate + (2S)-2,3-diaminopropionate
-
729202
Pantoea agglomerans
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
-
-
?
ATP + fumarate + (2S)-2,3-diaminopropionate
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
729202
Pantoea agglomerans
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
-
-
?
ATP + succinate + (2S)-2,3-diaminopropionate
activity with succinate is less than 5% of the activity compared to fumarate
729202
Pantoea agglomerans
?
-
-
-
?
additional information
no activity with the half-amides fumaramate and succinamate succinamate, or with the two enantiomers of trans-epoxysuccinate
729202
Pantoea agglomerans
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Pantoea agglomerans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8
-
succinate
pH 8.0, 22C
Pantoea agglomerans
11.6
-
fumarate
pH 8.0, 22C
Pantoea agglomerans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pantoea agglomerans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pantoea agglomerans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.55
-
fumarate
pH 8.0, 22C
Pantoea agglomerans
20
-
succinate
pH 8.0, 22C
Pantoea agglomerans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + fumarate + (2S)-2,3-diaminopropionate
Pantoea agglomerans
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pantoea agglomerans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + fumaramate + (2S)-2,3-diaminopropionate
activity with fumaramate is about 15% of the activity compared to fumarate
729202
Pantoea agglomerans
?
-
-
-
?
ATP + fumarate + (2S)-2,3-diaminopropionate
-
729202
Pantoea agglomerans
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
-
-
?
ATP + fumarate + (2S)-2,3-diaminopropionate
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
729202
Pantoea agglomerans
AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
-
-
?
ATP + succinate + (2S)-2,3-diaminopropionate
activity with succinate is less than 5% of the activity compared to fumarate
729202
Pantoea agglomerans
?
-
-
-
?
additional information
no activity with the half-amides fumaramate and succinamate succinamate, or with the two enantiomers of trans-epoxysuccinate
729202
Pantoea agglomerans
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
22
-
assay at
Pantoea agglomerans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.8
-
succinate
pH 8.0, 22C
Pantoea agglomerans
11.6
-
fumarate
pH 8.0, 22C
Pantoea agglomerans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pantoea agglomerans
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
Pantoea agglomerans
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold)
Pantoea agglomerans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.19
-
succinate
pH 8.0, 22C
Pantoea agglomerans
21
-
fumarate
pH 8.0, 22C
Pantoea agglomerans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.19
-
succinate
pH 8.0, 22C
Pantoea agglomerans
21
-
fumarate
pH 8.0, 22C
Pantoea agglomerans
Other publictions for EC 6.3.2.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730234
Dawlaty
Dapdiamides, tripeptide antibi ...
Pantoea agglomerans
J. Nat. Prod.
73
441-446
2010
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1
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729202
Hollenhorst
The ATP-dependent amide ligase ...
Pantoea agglomerans
Biochemistry
48
10467-10472
2009
-
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1
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2
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1
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1
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1
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5
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1
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2
1
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1
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2
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1
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1
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5
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1
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2
1
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1
1
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2
2