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Literature summary for 6.3.2.46 extracted from

  • Hollenhorst, M.A.; Clardy, J.; Walsh, C.T.
    The ATP-dependent amide ligases DdaG and DdaF assemble the fumaramoyl-dipeptide scaffold of the dapdiamide antibiotics (2009), Biochemistry, 48, 10467-10472.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.46

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea agglomerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
 fumarate pH 8.0, 22°C Pantoea agglomerans
20
-
 succinate pH 8.0, 22°C Pantoea agglomerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + fumarate + (2S)-2,3-diaminopropionate Pantoea agglomerans the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold) AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
 ?

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans E2JA32
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + fumaramate + (2S)-2,3-diaminopropionate activity with fumaramate is about 15% of the activity compared to fumarate Pantoea agglomerans ?
-
 ?
ATP + fumarate + (2S)-2,3-diaminopropionate
-
 Pantoea agglomerans AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
 ?
ATP + fumarate + (2S)-2,3-diaminopropionate the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold) Pantoea agglomerans AMP + diphosphate + (2E)-4-[(2-amino-2-carboxyethyl)amino]-4-oxobut-2-enoate
-
 ?
ATP + succinate + (2S)-2,3-diaminopropionate activity with succinate is less than 5% of the activity compared to fumarate Pantoea agglomerans ?
-
 ?
additional information no activity with the half-amides fumaramate and succinamate succinamate, or with the two enantiomers of trans-epoxysuccinate Pantoea agglomerans ?
-
 ?

Synonyms

Synonyms Comment Organism
DdaG
-
 Pantoea agglomerans
fumaroyl-2,3-diaminopropionate ligase
-
 Pantoea agglomerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Pantoea agglomerans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.8
-
 succinate pH 8.0, 22°C Pantoea agglomerans
11.6
-
 fumarate pH 8.0, 22°C Pantoea agglomerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pantoea agglomerans

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of dapdiamide antibiotics (a family of fumaramoyl- and epoxysuccinamoyl-dipeptides, named for the presence of 2,3-diaminopropionate (DAP) and the two amide linkages of the antibiotic scaffold) Pantoea agglomerans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 succinate pH 8.0, 22°C Pantoea agglomerans
21
-
 fumarate pH 8.0, 22°C Pantoea agglomerans