BRENDA - Enzyme Database show
show all sequences of 6.3.2.44

A novel isoform of pantothenate synthetase in the Archaea

Ronconi, S.; Jonczyk, R.; Genschel, U.; FEBS J. 275, 2754-2764 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli as a N-terminally His-tag fusion protein
Methanosarcina mazei
Inhibitors
Inhibitors
Commentary
Organism
Structure
(R)-pantothenate
strong product inhibition
Methanosarcina mazei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Methanosarcina mazei
57000
-
gel filtration
Methanosarcina mazei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-pantoate + beta-alanine
Methanosarcina mazei
the enzyme is involved in the biosynthesis of pantothenate
ADP + phosphate + (R)-pantothenate
-
-
?
ATP + (R)-pantoate + beta-alanine
Methanosarcina mazei DSM 3647
the enzyme is involved in the biosynthesis of pantothenate
ADP + phosphate + (R)-pantothenate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanosarcina mazei
Q8PUQ1
-
-
Methanosarcina mazei DSM 3647
Q8PUQ1
-
-
Purification (Commentary)
Commentary
Organism
-
Methanosarcina mazei
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.14
-
pH 8.0, 37C
Methanosarcina mazei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-pantoate + beta-alanine
the enzyme is involved in the biosynthesis of pantothenate
727465
Methanosarcina mazei
ADP + phosphate + (R)-pantothenate
-
-
-
?
ATP + (R)-pantoate + beta-alanine
purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase
727465
Methanosarcina mazei
ADP + phosphate + (R)-pantothenate
-
-
-
?
ATP + (R)-pantoate + beta-alanine
the enzyme is involved in the biosynthesis of pantothenate
727465
Methanosarcina mazei DSM 3647
ADP + phosphate + (R)-pantothenate
-
-
-
?
ATP + (R)-pantoate + beta-alanine
purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase
727465
Methanosarcina mazei DSM 3647
ADP + phosphate + (R)-pantothenate
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Methanosarcina mazei
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Methanosarcina mazei
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Methanosarcina mazei
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli as a N-terminally His-tag fusion protein
Methanosarcina mazei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R)-pantothenate
strong product inhibition
Methanosarcina mazei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
2 * 30000, SDS-PAGE
Methanosarcina mazei
57000
-
gel filtration
Methanosarcina mazei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-pantoate + beta-alanine
Methanosarcina mazei
the enzyme is involved in the biosynthesis of pantothenate
ADP + phosphate + (R)-pantothenate
-
-
?
ATP + (R)-pantoate + beta-alanine
Methanosarcina mazei DSM 3647
the enzyme is involved in the biosynthesis of pantothenate
ADP + phosphate + (R)-pantothenate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanosarcina mazei
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.14
-
pH 8.0, 37C
Methanosarcina mazei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + (R)-pantoate + beta-alanine
the enzyme is involved in the biosynthesis of pantothenate
727465
Methanosarcina mazei
ADP + phosphate + (R)-pantothenate
-
-
-
?
ATP + (R)-pantoate + beta-alanine
purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase
727465
Methanosarcina mazei
ADP + phosphate + (R)-pantothenate
-
-
-
?
ATP + (R)-pantoate + beta-alanine
the enzyme is involved in the biosynthesis of pantothenate
727465
Methanosarcina mazei DSM 3647
ADP + phosphate + (R)-pantothenate
-
-
-
?
ATP + (R)-pantoate + beta-alanine
purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase
727465
Methanosarcina mazei DSM 3647
ADP + phosphate + (R)-pantothenate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 30000, SDS-PAGE
Methanosarcina mazei
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Methanosarcina mazei
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Methanosarcina mazei
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of pantothenate
Methanosarcina mazei
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of pantothenate
Methanosarcina mazei
Other publictions for EC 6.3.2.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727465
Ronconi
A novel isoform of pantothenat ...
Methanosarcina mazei, Methanosarcina mazei DSM 3647
FEBS J.
275
2754-2764
2008
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1
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1
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1
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1
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2
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1
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1
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4
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1
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1
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