Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a N-terminally His-tag fusion protein | Methanosarcina mazei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-pantothenate | strong product inhibition | Methanosarcina mazei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, SDS-PAGE | Methanosarcina mazei |
57000 | - |
gel filtration | Methanosarcina mazei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | Methanosarcina mazei | the enzyme is involved in the biosynthesis of pantothenate | ADP + phosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | Methanosarcina mazei DSM 3647 | the enzyme is involved in the biosynthesis of pantothenate | ADP + phosphate + (R)-pantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | Q8PUQ1 | - |
- |
Methanosarcina mazei DSM 3647 | Q8PUQ1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanosarcina mazei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
pH 8.0, 37°C | Methanosarcina mazei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantoate + beta-alanine | the enzyme is involved in the biosynthesis of pantothenate | Methanosarcina mazei | ADP + phosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase | Methanosarcina mazei | ADP + phosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | the enzyme is involved in the biosynthesis of pantothenate | Methanosarcina mazei DSM 3647 | ADP + phosphate + (R)-pantothenate | - |
? | |
ATP + (R)-pantoate + beta-alanine | purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase | Methanosarcina mazei DSM 3647 | ADP + phosphate + (R)-pantothenate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, SDS-PAGE | Methanosarcina mazei |
Synonyms | Comment | Organism |
---|---|---|
MM2281 | locus name | Methanosarcina mazei |
Pantothenate synthetase | ambiguous | Methanosarcina mazei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina mazei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Methanosarcina mazei |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of pantothenate | Methanosarcina mazei |