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Literature summary for 6.3.2.44 extracted from

  • Ronconi, S.; Jonczyk, R.; Genschel, U.
    A novel isoform of pantothenate synthetase in the Archaea (2008), FEBS J., 275, 2754-2764.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a N-terminally His-tag fusion protein Methanosarcina mazei

Inhibitors

Inhibitors Comment Organism Structure
(R)-pantothenate strong product inhibition Methanosarcina mazei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, SDS-PAGE Methanosarcina mazei
57000
-
gel filtration Methanosarcina mazei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-pantoate + beta-alanine Methanosarcina mazei the enzyme is involved in the biosynthesis of pantothenate ADP + phosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine Methanosarcina mazei DSM 3647 the enzyme is involved in the biosynthesis of pantothenate ADP + phosphate + (R)-pantothenate
-
?

Organism

Organism UniProt Comment Textmining
Methanosarcina mazei Q8PUQ1
-
-
Methanosarcina mazei DSM 3647 Q8PUQ1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanosarcina mazei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
pH 8.0, 37°C Methanosarcina mazei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate + beta-alanine the enzyme is involved in the biosynthesis of pantothenate Methanosarcina mazei ADP + phosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase Methanosarcina mazei ADP + phosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine the enzyme is involved in the biosynthesis of pantothenate Methanosarcina mazei DSM 3647 ADP + phosphate + (R)-pantothenate
-
?
ATP + (R)-pantoate + beta-alanine purified enzyme shows no activity on its own, but the enzyme enables substantial synthesis of 4'-phosphopantothenate from beta-alanine, pantoate and ATP when coupled with Escherichia coli pantothenate kinase Methanosarcina mazei DSM 3647 ADP + phosphate + (R)-pantothenate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Methanosarcina mazei

Synonyms

Synonyms Comment Organism
MM2281 locus name Methanosarcina mazei
Pantothenate synthetase ambiguous Methanosarcina mazei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanosarcina mazei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Methanosarcina mazei

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of pantothenate Methanosarcina mazei