Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
[LysW]-C-terminal-L-glutamate54 | pH 8.0, 60°C | Thermus thermophilus | |
2.1 | - |
L-2-aminoadipate | pH 8.0, 60°C | Thermus thermophilus | |
2.6 | - |
ATP | pH 8.0, 60°C | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate | Thermus thermophilus | the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically | ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SH23 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate | the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically | Thermus thermophilus | ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54 | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate | LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically | Thermus thermophilus | ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-aminoadipate-lysW ligase lysX | - |
Thermus thermophilus |
LysX | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
L-2-aminoadipate | pH 8.0, 60°C | Thermus thermophilus | |
2 | - |
[LysW]-C-terminal-L-glutamate54 | pH 8.0, 60°C | Thermus thermophilus | |
5.2 | - |
ATP | pH 8.0, 60°C | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of lysine in Thermus thermophilus | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-2-aminoadipate | pH 8.0, 60°C | Thermus thermophilus | |
2.7 | - |
ATP | pH 8.0, 60°C | Thermus thermophilus | |
26 | - |
[LysW]-C-terminal-L-glutamate54 | pH 8.0, 60°C | Thermus thermophilus |