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Literature summary for 6.3.2.43 extracted from

  • Horie, A.; Tomita, T.; Saiki, A.; Kono, H.; Taka, H.; Mineki, R.; Fujimura, T.; Nishiyama, C.; Kuzuyama, T.; Nishiyama, M.
    Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus (2009), Nat. Chem. Biol., 5, 673-679.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
[LysW]-C-terminal-L-glutamate54 pH 8.0, 60°C Thermus thermophilus
2.1
-
L-2-aminoadipate pH 8.0, 60°C Thermus thermophilus
2.6
-
ATP pH 8.0, 60°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate Thermus thermophilus the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SH23
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate the enzyme is involved in biosynthesis of lysine. LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically Thermus thermophilus ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54
-
?
ATP + [LysW]-C-terminal-L-glutamate54 + L-2-aminoadipate LysX activates the gamma-carboxyl group of the C-terminal Glu54 of the 2-L-aminoaadipate carrier-protein LysW by phosphorylation, with concomitant conversion of ATP to ADP. LysX recognizes not only the C-terminal glutamate residue of LysW but also other portions, possibly the globular domain of LysW specifically Thermus thermophilus ADP + phosphate + [LysW]-C-terminal-gamma-(L-2-aminoadip-2-yl)-L-glutamate54
-
?

Synonyms

Synonyms Comment Organism
alpha-aminoadipate-lysW ligase lysX
-
Thermus thermophilus
LysX
-
Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.85
-
L-2-aminoadipate pH 8.0, 60°C Thermus thermophilus
2
-
[LysW]-C-terminal-L-glutamate54 pH 8.0, 60°C Thermus thermophilus
5.2
-
ATP pH 8.0, 60°C Thermus thermophilus

General Information

General Information Comment Organism
physiological function the enzyme is involved in biosynthesis of lysine in Thermus thermophilus Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
L-2-aminoadipate pH 8.0, 60°C Thermus thermophilus
2.7
-
ATP pH 8.0, 60°C Thermus thermophilus
26
-
[LysW]-C-terminal-L-glutamate54 pH 8.0, 60°C Thermus thermophilus