BRENDA - Enzyme Database show
show all sequences of 6.3.2.42

N-Acetylaspartylglutamate synthetase II synthesizes N-acetylaspartylglutamylglutamate

Lodder-Gadaczek, J.; Becker, I.; Gieselmann, V.; Wang-Eckhardt, L.; Eckhardt, M.; J. Biol. Chem. 286, 16693-16706 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed as FLAG epitope-tagged protein in HEK-293T and CHO-K1 cells; expressed as FLAG epitope-tagged protein in HEK-293T and CHO-K1 cells. Because hexahistidine-tagged Escherichia coli-expressed enzyme using bacterial expressed protein
Mus musculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
;
Mus musculus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
x * 42000, SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
Mus musculus
-
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
?
ATP + N-acetyl-L-aspartate + L-glutamate
Mus musculus
-
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
-
-
-
Mus musculus
Q6PFX8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
brain stem
;
Mus musculus
-
central nervous system
highest expression levels in the brain stem and spinal cord, almost undetectable in the neocortex
Mus musculus
-
cerebellum
; strong expression is restricted to deep cerebellar nuclei
Mus musculus
-
forebrain
-
Mus musculus
-
kidney
-
Mus musculus
-
sciatic nerve
-
Mus musculus
-
spinal cord
; expression is found in the ventral and dorsal horns of gray matter in laminae III to X, but is low or absent in laminae I and II
Mus musculus
-
testis
-
Mus musculus
-
thymus
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
-
725423
Mus musculus
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
-
?
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
the enzyme also has the activity of EC 6.3.2.41, N-acetylaspartylglutamate synthase
725423
Mus musculus
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
-
?
ATP + N-acetyl-L-aspartate + L-glutamate
-
725423
Mus musculus
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed as FLAG epitope-tagged protein in HEK-293T and CHO-K1 cells
Mus musculus
expressed as FLAG epitope-tagged protein in HEK-293T and CHO-K1 cells. Because hexahistidine-tagged Escherichia coli-expressed enzyme using bacterial expressed protein
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Mus musculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mus musculus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
x * 42000, SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
Mus musculus
-
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
?
ATP + N-acetyl-L-aspartate + L-glutamate
Mus musculus
-
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Mus musculus
-
brain stem
-
Mus musculus
-
central nervous system
highest expression levels in the brain stem and spinal cord, almost undetectable in the neocortex
Mus musculus
-
cerebellum
-
Mus musculus
-
cerebellum
strong expression is restricted to deep cerebellar nuclei
Mus musculus
-
forebrain
-
Mus musculus
-
kidney
-
Mus musculus
-
sciatic nerve
-
Mus musculus
-
spinal cord
-
Mus musculus
-
spinal cord
expression is found in the ventral and dorsal horns of gray matter in laminae III to X, but is low or absent in laminae I and II
Mus musculus
-
testis
-
Mus musculus
-
thymus
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
-
725423
Mus musculus
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
-
?
2 ATP + N-acetyl-L-aspartate + 2 L-glutamate
the enzyme also has the activity of EC 6.3.2.41, N-acetylaspartylglutamate synthase
725423
Mus musculus
2 ADP + 2 phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
-
?
ATP + N-acetyl-L-aspartate + L-glutamate
-
725423
Mus musculus
ADP + phosphate + N-acetyl-L-aspartyl-L-glutamyl-L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 42000, SDS-PAGE
Mus musculus
Other publictions for EC 6.3.2.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725423
Lodder-Gadaczek
N-Acetylaspartylglutamate synt ...
Mus musculus
J. Biol. Chem.
286
16693-16706
2011
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1
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1
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1
2
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2
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10
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3
1
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1
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2
1
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2
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1
2
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13
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3
1
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