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Literature summary for 6.3.2.41 extracted from

  • Collard, F.; Stroobant, V.; Lamosa, P.; Kapanda, C.N.; Lambert, D.M.; Muccioli, G.G.; Poupaert, J.H.; Opperdoes, F.; van Schaftingen, E.
    Molecular identification of N-acetylaspartylglutamate synthase and beta-citrylglutamate synthase (2010), J. Biol. Chem., 285, 29826-29833.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.41

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulates Mus musculus
dithiothreitol the enzyme is markedly stimulated by dithiothreitol, which increases the activity by about 5fold Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in bacteria or HEK293T cells Mus musculus
expressed in Escherichia coli Mus musculus
expressed in Escherichia coli BL21(DE3)pLysS cells and in HEK-293T cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 ATP pH 8.0, 30°C Mus musculus
0.065
-
 ATP pH 8.0, 30°C Mus musculus
0.065
-
 ATP with 5 mM MgATP, at pH 8.0 and 37°C Mus musculus
0.73
-
 L-glutamate pH 8.0, 30°C Mus musculus
0.87
-
 citrate at pH 8.0 and 37°C Mus musculus
0.88
-
 L-glutamate pH 8.0, 30°C Mus musculus
0.88
-
 L-glutamate at pH 8.0 and 37°C Mus musculus
1.48
-
 N-acetyl-L-aspartate pH 8.0, 30°C Mus musculus
1.48
-
 N-acetyl-L-aspartate at pH 8.0 and 37°C Mus musculus
4.59
-
 N-acetyl-L-aspartate pH 8.0, 30°C Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, SDS-PAGE Mus musculus
43000
-
 x * 43000, SDS-PAGE Mus musculus
43000
-
 x * 43000, contains at least six subunits, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N-acetyl-L-aspartate + L-glutamate Mus musculus
-
 ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q6PFX8
-
-
Mus musculus Q80WS1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and S-200 gel filtration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system
-
 Mus musculus
-
dorsal root ganglion
-
 Mus musculus
-
placenta
-
 Mus musculus
-
spinal cord
-
 Mus musculus
-
testis
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.09
-
 pH 8.0, 30°C, formation of N-acetyl-L-aspartyl-L-glutamate Mus musculus
1.4
-
 pH 8.0, 30°C, formation of N-acetyl-L-aspartyl-L-glutamate Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-aspartate + citrate
-
 Mus musculus ?
-
 ?
ATP + N-acetyl-L-aspartate + L-glutamate
-
 Mus musculus ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate
-
 ?
ATP + N-acetyl-L-aspartate + L-glutamate isoform RIMKLA also catalyses the synthesis of beta-citryl-L-glutamate with an activity that is 75fold lower than its N-acetyl-L-aspartyl-L-glutamate synthase activity Mus musculus ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate
-
 ?
ATP + N-acetyl-L-aspartate + L-glutamate isoform RIMKLB catalyses the synthesis of beta-citryl-L-glutamate and N-acetyl-L-aspartyl-L-glutamate at nearly equal rates, cf. EC 6.3.1.17, beta-citrylglutamate synthase Mus musculus ADP + phosphate + N-acetyl-L-aspartyl-L-glutamate
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Mus musculus
? x * 43000, SDS-PAGE Mus musculus
? x * 43000, contains at least six subunits, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
N-acetylaspartylglutamate synthetase
-
 Mus musculus
NAAG synthetase
-
 Mus musculus
NAAGS
-
 Mus musculus
RIMKLA
-
 Mus musculus
RIMKLA gene name, ambiguous Mus musculus
RIMKLB gene name, ambiguous Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 N-acetyl-L-aspartate pH 8.0, 30°C Mus musculus
2.6
-
 N-acetyl-L-aspartate at pH 8.0 and 37°C Mus musculus
3
-
 N-acetyl-L-aspartate pH 8.0, 30°C Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Mus musculus
8
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus