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Literature summary for 6.3.2.4 extracted from

  • Nakagawa, T.; Satake, R.; Sato, M.; Kino, K.
    Structure-based modification of D-alanine-D-alanine ligase from Thermotoga maritima ATCC 43589 for depsipeptide synthesis (2011), Biosci. Biotechnol. Biochem., 75, 700-704.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
S137A/Y207F higher D-alanyl-D-lactate and lower D-alanyl-D-alanine synthase activity than mutant Y207F. Broad substrate specificity toward D-amino acids, accepts D-lactate and D,L-isoleucine as substrate Thermotoga maritima
S137F/Y207F about 20% of wild-type activity. Accepts D-lactate as substrate Thermotoga maritima
S137G/Y207F about 200% of wild-type activity. Accepts D-lactate as substrate Thermotoga maritima
S137T/Y207F about 40% of wild-type activity. Does not accept D-lactate as substrate Thermotoga maritima
Y201F increase in activity compared to wild-type, mutant is not able to synthesize D-alanyl-D-lactate Thermotoga maritima
Y207F mutant is able to synthesize D-alanyl-D-lactate Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima ATCC 43589
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
mutant S137F/Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 Thermotoga maritima
0.39
-
mutant Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 Thermotoga maritima
0.42
-
mutant S137A/Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 Thermotoga maritima
0.95
-
mutant S137F/Y207F, substrate D-Ala, 60°C, pH 7.5 Thermotoga maritima
1.2
-
mutant S137G/Y207F, substrates D-Ala + D-lactate, 60°C, pH 7.5 Thermotoga maritima
1.75
-
mutant S137T/Y207F, substrate D-Ala, 60°C, pH 7.5 Thermotoga maritima
4.22
-
wild-type, substrate D-Ala, 60°C, pH 7.5 Thermotoga maritima
6.489
-
mutant S137A/Y207F, substrate D-Ala, 60°C, pH 7.5 Thermotoga maritima
9.66
-
mutant S137G/Y207F, substrate D-Ala, 60°C, pH 7.5 Thermotoga maritima
17.22
-
mutant Y207F, substrate D-Ala, 60°C, pH 7.5 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-Ala + D-Ala
-
Thermotoga maritima ADP + phosphate + D-Ala-D-Ala
-
?
ATP + D-Ala + D-Ala
-
Thermotoga maritima ATCC 43589 ADP + phosphate + D-Ala-D-Ala
-
?
ATP + D-Ala + D-lactate D-lactate is not a substrate for wild-type, but for mutants S137A/Y207F, S137F/Y207F, S137T/Y207F, S137G/Y207F, Y207F Thermotoga maritima ADP + phosphate + D-Ala-D-lactate
-
?
ATP + D-Ala + D-lactate D-lactate is not a substrate for wild-type, but for mutants S137A/Y207F, S137F/Y207F, S137T/Y207F, S137G/Y207F, Y207F Thermotoga maritima ATCC 43589 ADP + phosphate + D-Ala-D-lactate
-
?