Cloned (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus DSM 4304 |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the enzyme from Archaeoglobus fulgidus and its complex with GDP at 2.5 A and 1.35 A resolution, respectively. CofE-AF crystallization is performed by the sitting-drop and hanging-drop methods using vapor diffusion at 18°C | Archaeoglobus fulgidus DSM 4304 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | there are two Mn2+-binding sites per monomer within close proximity of the GDP alpha and beta-phosphate groups | Archaeoglobus fulgidus DSM 4304 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + GTP + coenzyme F420-1 | Archaeoglobus fulgidus DSM 4304 | the enzyme protein catalyzes two distinct and independent reactions, firstly attaching a glutamte via its alpha-NH2 to F420-0 (cf. coenzyme F420-0:glutamyl ligase). The second reaction is a gamma ligation, taking place when a certain amount of monoglutamylated F420-1 has accumulated | GDP + phosphate + coenzyme gamma-F420-2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus DSM 4304 | O28028 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus DSM 4304 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + GTP + coenzyme F420-1 | the enzyme protein catalyzes two distinct and independent reactions, firstly attaching a glutamte via its alpha-NH2 to F420-0 (cf. coenzyme F420-0:glutamyl ligase). The second reaction is a gamma ligation, taking place when a certain amount of monoglutamylated F420-1 has accumulated | Archaeoglobus fulgidus DSM 4304 | GDP + phosphate + coenzyme gamma-F420-2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CofE-AF | - |
Archaeoglobus fulgidus DSM 4304 |
F420:gamma-glutamyl ligase | family of non-ribosomal peptide synthases | Archaeoglobus fulgidus DSM 4304 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Archaeoglobus fulgidus DSM 4304 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Archaeoglobus fulgidus DSM 4304 |