BRENDA - Enzyme Database
show all sequences of 6.3.2.33

Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses

Li, H.; Xu, H.; Graham, D.E.; White, R.H.; Proc. Natl. Acad. Sci. USA 100, 9785-9790 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
conserved Mg-ATP-binding domain
Methanocaldococcus jannaschii
additional information
does not require K+ for activity
Methanocaldococcus jannaschii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33278
-
2 * 33278, calculated from sequence
Methanocaldococcus jannaschii
35000
-
2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
72000
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Methanocaldococcus jannaschii
Q58037
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
12
-
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of ATP and K+
Methanocaldococcus jannaschii
12.7
-
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of GTP and K+
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
ATP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 33278, calculated from sequence; 2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
Synonyms
Synonyms
Commentary
Organism
H4MPT:alpha-L-glutamate ligase
-
Methanocaldococcus jannaschii
MJ0620
-
Methanocaldococcus jannaschii
MptN protein
-
Methanocaldococcus jannaschii
tetrahydromethanopterin:alpha-L-glutamate ligase
-
Methanocaldococcus jannaschii
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
60
-
assay at
Methanocaldococcus jannaschii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
conserved Mg-ATP-binding domain
Methanocaldococcus jannaschii
additional information
does not require K+ for activity
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33278
-
2 * 33278, calculated from sequence
Methanocaldococcus jannaschii
35000
-
2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
72000
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
Methanocaldococcus jannaschii
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanocaldococcus jannaschii
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
12
-
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of ATP and K+
Methanocaldococcus jannaschii
12.7
-
under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of GTP and K+
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
ATP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
GTP + tetrahydromethanopterin + L-glutamate
the enzyme does not discriminate between ATP and GTP
700925
Methanocaldococcus jannaschii
GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 33278, calculated from sequence; 2 * 35000, SDS-PAGE
Methanocaldococcus jannaschii
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
60
-
assay at
Methanocaldococcus jannaschii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Methanocaldococcus jannaschii
Other publictions for EC 6.3.2.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700925
Li
Glutathione synthetase homolog ...
Methanocaldococcus jannaschii
Proc. Natl. Acad. Sci. USA
100
9785-9790
2003
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4
1
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1
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2
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2
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1
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1
1
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1
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