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Literature summary for 6.3.2.33 extracted from

  • Li, H.; Xu, H.; Graham, D.E.; White, R.H.
    Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses (2003), Proc. Natl. Acad. Sci. USA, 100, 9785-9790.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.33

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ conserved Mg-ATP-binding domain Methanocaldococcus jannaschii
additional information does not require K+ for activity Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33278
-
 2 * 33278, calculated from sequence Methanocaldococcus jannaschii
35000
-
 2 * 35000, SDS-PAGE Methanocaldococcus jannaschii
72000
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + tetrahydromethanopterin + L-glutamate Methanocaldococcus jannaschii biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
 ?
GTP + tetrahydromethanopterin + L-glutamate Methanocaldococcus jannaschii biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58037
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of ATP and K+ Methanocaldococcus jannaschii
12.7
-
 under rate-limiting assay conditions, MptN produces 12.7 nmol of sarcinapterin per min per mg of protein in the presence of GTP and K+ Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + tetrahydromethanopterin + L-glutamate biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP Methanocaldococcus jannaschii ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
 ?
ATP + tetrahydromethanopterin + L-glutamate the enzyme does not discriminate between ATP and GTP Methanocaldococcus jannaschii ADP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
 ?
GTP + tetrahydromethanopterin + L-glutamate biosynthesis of the cofactor 5,6,7,8-tetrahydrosarcinapterin, the enzyme does not discriminate between ATP and GTP Methanocaldococcus jannaschii GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
 ?
GTP + tetrahydromethanopterin + L-glutamate the enzyme does not discriminate between ATP and GTP Methanocaldococcus jannaschii GDP + phosphate + 5,6,7,8-tetrahydrosarcinapterin
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Methanocaldococcus jannaschii
homodimer 2 * 33278, calculated from sequence Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
H4MPT:alpha-L-glutamate ligase
-
 Methanocaldococcus jannaschii
MJ0620
-
 Methanocaldococcus jannaschii
MptN protein
-
 Methanocaldococcus jannaschii
tetrahydromethanopterin:alpha-L-glutamate ligase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 assay at Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methanocaldococcus jannaschii