Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.30 extracted from

  • Hai, T.; Oppermann-Sanio, F.B.; Steinbüchel, A.
    Molecular characterization of a thermostable cyanophycin synthetase from the thermophilic cyanobacterium Synechococcus sp. strain MA19 and in vitro synthesis of cyanophycin and related polyamides (2002), Appl. Environ. Microbiol., 68, 93-101.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli TOP10 cells Synechococcus sp. MA19

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 monomer, SDS-PAGE Synechococcus sp. MA19
100600
-
 monomer, calculated from the amino acid sequence Synechococcus sp. MA19

Organism

Organism UniProt Comment Textmining
Synechococcus sp. MA19 Q8VTA5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Synechococcus sp. MA19

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the activity EC 6.3.2.29 Synechococcus sp. MA19
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.113
-
 30°C, pH 8.2, 123fold purification Synechococcus sp. MA19

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Synechococcus sp. MA19 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
additional information negligible activities when L-aspartic acid is omitted Synechococcus sp. MA19 ?
-
 ?

Synonyms

Synonyms Comment Organism
CphA
-
 Synechococcus sp. MA19
cyanophycin synthetase
-
 Synechococcus sp. MA19

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Synechococcus sp. MA19

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 preincubation for 30 min in this temperature range results in 84% to 72% activity compared to preincubation at 30°C Synechococcus sp. MA19